Protocol abstract
Nature Protocols 1, - 2988 - 2994 (2007)
Published online: 25 January 2007 | doi:10.1038/nprot.2006.376
Subject Categories: Imaging | Model organisms
Whole-body imaging of bacterial infection and antibiotic response
Robert M Hoffman1,2 & Ming Zhao1
Abstract
We describe imaging of green fluorescent protein (GFP)-expressing bacteria from outside intact infected animals. This simple, non-intrusive technique can show in great detail the spatial–temporal behavior of the infectious process. The bacteria, expressing the GFP, are sufficiently bright as to be clearly visible from outside the infected animal and recorded with simple equipment. Introduced bacteria can be whole-body imaged in most mouse organs, including the peritoneal cavity, stomach, small intestine, and colon. This imaging technology affords a powerful approach to visualizing the infection process, determining the tissue specificity of infection, the spatial migration of the infectious agents and the response to antimicrobial agents.
- AntiCancer Incorporated, 7917 Ostrow Street, San Diego, California, 92111, USA.
- Department of Surgery, University of California at San Diego, 200 West Arbor Drive, San Diego,California, 92103-8220, USA.
Correspondence to: Robert M Hoffman1,2 e-mail: all@anticancer.com
nature-products
A-Z product listing
- autoclaved laboratory rodent diet(Tecklad)
- Blue LED flashlight(LDP)
- cells(Clontech)
- Culture dishes(Fisher Scientific)
- D470/40 excitation filter(Chroma Technology)
- DP70 CCD camera(Olympus Corporation)
