The mid-infrared spectral region of 2-20 μm contains strong characteristic vibrational transitions of many important molecules as well as two atmospheric transmission windows of 3-5 μm and 8-13 μm, which makes it crucial for applications in spectroscopy, materials processing, chemical and biomolecular sensing, security and industry. This Focus Issue covers recent developments in sophisticated laser technologies such as quantum cascade lasers, fibre lasers and frequency combs, and discusses how plasmonics can be exploited in the mid-infrared regime.