Volume 8 Issue 9, September 2011

Editorial

Building a better mouse test

As more mouse models are produced, researchers studying neuropsychiatric diseases will need better ways to evaluate them and more realistic assessment of the results.

Editorial 30 Aug 2011

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This Month

Greg Davis

Gene editing becomes faster and more facile.

Monya Baker
Greg Davis
This Month 30 Aug 2011
Arrows

Bang Wong
This Month 30 Aug 2011

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Correspondence

TopFIND, a knowledgebase linking protein termini with function

Philipp F Lange
Christopher M Overall
Correspondence 07 Aug 2011

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Research Highlights

More options for gene editing

Engineered nucleases work across species and in isogenic stem cells.

Monya Baker
Research Highlights 30 Aug 2011
Speed-reading DNA in the dark

A new chip with a capability to read genomes uses arrays of tiny pH sensors on an integrated circuit to sequence DNA without the need for optics.

Tal Nawy
Research Highlights 30 Aug 2011
Watching neurotransmission in vitro

An in vitro system for neurotransmitter release is reported.

Natalie de Souza
Research Highlights 30 Aug 2011

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News in Brief

Large-scale genome editing in Escherichia coli

News in Brief 30 Aug 2011
Clickable, stable fluorophores

News in Brief 30 Aug 2011
Optogenetic tools for social disorders

News in Brief 30 Aug 2011
High-resolution structures from low-resolution data

News in Brief 30 Aug 2011
RNA scaffolds for biosynthesis in bugs

News in Brief 30 Aug 2011

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Research Highlights

Regulation through degradation

Two groups present small molecule–induced degradation systems for controlling protein function in living systems.

Allison Doerr
Research Highlights 30 Aug 2011
Everything in its right place

Clever design strategies enable researchers to harness nonspecific interactions to achieve highly specific arrangements of nucleic acid nanostructures.

Michael Eisenstein
Research Highlights 30 Aug 2011
Bacterial electrophysiology brought to light

An engineered fluorescent protein reveals electrical spikes in intact bacterial cells.

Petya V Krasteva
Research Highlights 30 Aug 2011

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Technology Feature

Making sense of chromatin states

Researchers find new pieces in the puzzle of genome regulation.

Monya Baker
Technology Feature 30 Aug 2011

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News & Views

On target? Tracing zinc-finger-nuclease specificity

In two independent studies, researchers experimentally test the cleavage specificity of zinc-finger nucleases across the genome.

Claudio Mussolino
Toni Cathomen
News & Views 30 Aug 2011
Beyond the rainbow: new fluorescent proteins brighten the infrared scene

Two fluorescent proteins that emit in the far-red and infrared range for imaging applications in cells and in vivo are described.

Michael Z Lin
News & Views 30 Aug 2011
'MiMICing' genomic flexibility

A new collection of Minos transposon insertions will enhance the range and flexibility of genome engineering in Drosophila melanogaster.

Steven Russell
News & Views 30 Aug 2011

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Perspective

Three-dimensional biomaterials for the study of human pluripotent stem cells

Thomas P Kraehenbuehl
Robert Langer
Lino S Ferreira
Perspective 30 Aug 2011

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Resource

MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes

Described is a randomly inserting transposon that can be swapped for gene traps, coding insertions or protein tag genes, thus expanding the toolkit for Drosophila melanogaster genome engineering.

Koen J T Venken
Karen L Schulze
Hugo J Bellen
Resource 31 Jul 2011
Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function

A collection of functionally characterized BAC transgenic mouse lines in which the channelrhodopsin-2 H134R variant is specifically and stably expressed in GABAergic, cholinergic, serotonergic or parvalbumin-positive neurons is reported.

Shengli Zhao
Jonathan T Ting
Guoping Feng
Resource 07 Aug 2011

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Brief Communication

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

Single-stranded oligonucleotides are used as donor templates for zinc-finger nucleases to create targeted genomic deletions and mutations in mammalian cell lines.

Fuqiang Chen
Shondra M Pruett-Miller
Gregory D Davis
Brief Communication 17 Jul 2011
Deep and fast live imaging with two-photon scanned light-sheet microscopy

Two-photon excitation in scanned light-sheet microscopy allows deeper imaging than the one-photon implementation and faster three-dimensional imaging of organism development with no evidence of phototoxicity compared to conventional two-photon point scanning microscopy.

Thai V Truong
Willy Supatto
Scott E Fraser
Brief Communication 17 Jul 2011
Bayesian community-wide culture-independent microbial source tracking

SourceTracker finds the proportion and origin of contaminants in a given sample. Its database will prove useful in screening of metagenomic datasets for contaminants.

Dan Knights
Justin Kuczynski
Scott T Kelley
Brief Communication 17 Jul 2011

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Article

Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

An in vitro method for examining cleavage patterns of zinc-finger nucleases (ZFNs) identifies previously unknown off-target cleavage sites. Some of the sites are present in the human genome and show evidence for ZFN-induced cleavage in cultured human cell lines.

Vikram Pattanayak
Cherie L Ramirez
David R Liu
Article 07 Aug 2011
A photoswitchable orange-to-far-red fluorescent protein, PSmOrange

A fluorescent protein that can be photoswitched with visible light from orange to far-red is presented. The photoconverted form has the most red-shifted excitation peak of all GFP-like fluorescent proteins to date and should be useful for many imaging applications.

Oksana M Subach
George H Patterson
Vladislav V Verkhusha
Article 31 Jul 2011

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Advertising Feature: Application Note

ReliaPrep^™ Large Volume HT gDNA Isolation System: gDNA isolation from blood samples

Christine Helt
Eric Vincent
Cristopher Cowan
Advertising Feature: Application Note 30 Aug 2011
Sensitive detection of cytokines in 1-μl serum samples using Proseek^®

Fredrik Hjelm
Bonnie Tran
Simon Fredriksson
Advertising Feature: Application Note 30 Aug 2011

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Volume 8 Issue 9, September 2011

Editorial

This Month

Correspondence

Research Highlights

News in Brief

Research Highlights

Technology Feature

News & Views

Perspective

Resource

Brief Communication

Article

Advertising Feature: Application Note

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