Brief Communication abstract


Nature Methods 6, 593 - 595 (2009)
Published online: 20 July 2009 | doi:10.1038/nmeth.1354

Virtual terminator nucleotides for next-generation DNA sequencing

Jayson Bowers1, Judith Mitchell1, Eric Beer1, Philip R Buzby1, Marie Causey1, J William Efcavitch1, Mirna Jarosz1, Edyta Krzymanska-Olejnik1, Li Kung1, Doron Lipson1, Geoffrey M Lowman1, Subramanian Marappan1, Peter McInerney1, Adam Platt1, Atanu Roy1, Suhaib M Siddiqi1, Kathleen Steinmann1 & John F Thompson1

Top

We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.

Top
  1. Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.

Correspondence to: John F Thompson1 e-mail: jthompson@helicosbio.com



MORE ARTICLES LIKE THIS

These links to content published by NPG are automatically generated.

REVIEWS

Sequencing technologies ??? the next generation

Nature Reviews Genetics Review (01 Jan 2010)

RESEARCH

In vivo imaging of siRNA delivery and silencing in tumors

Nature Medicine Technical Report (01 Mar 2007)