Nature Methods
- 5, 491 - 505 (2008)
Published online: 29 May 2008; | doi:10.1038/nmeth.1218
Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopyKeir C Neuman1 & Attila Nagy21
Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, 50 South Drive, Bethesda, Maryland 20892, USA. 2
Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, 50 South Drive, Bethesda, Maryland 20892, USA.
Correspondence should be addressed to Keir C Neuman neumankc@mail.nih.gov Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
