Nature Methods
- 5, 531 - 533 (2008)
Published online: 13 April 2008; | doi:10.1038/nmeth.1203
Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studiesSamuel X Guo1, Frederic Bourgeois1, Trushal Chokshi2, Nicholas J Durr1, Massimo A Hilliard3, Nikos Chronis2 & Adela Ben-Yakar11
Department of Mechanical Engineering, The University of Texas at Austin, 204 E. Dean Keeton Street, Austin, Texas 78712, USA. 2
Department of Mechanical Engineering, University of Michigan, 2350 Hayward Street, Ann Arbor, Michigan 48109, USA. 3
Queensland Brain Institute, University of Queensland, Brisbane QLD 4072, Australia.
Correspondence should be addressed to Adela Ben-Yakar ben-yakar@mail.utexas.edu A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings. Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the absence of anesthetics.
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