Perspective abstract


Nature Methods 5, 1005 - 1010 (2008)
Published online: 25 November 2008 | doi:10.1038/nmeth.1270

A large genome center's improvements to the Illumina sequencing system

Michael A Quail1, Iwanka Kozarewa1, Frances Smith1, Aylwyn Scally1, Philip J Stephens1, Richard Durbin1, Harold Swerdlow1 & Daniel J Turner1


The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

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  1. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK.

Correspondence to: Daniel J Turner1 e-mail: djt@sanger.ac.uk.