Brief Communication abstract
Nature Methods 5, 955 - 957 (2008)
Published online: 26 October 2008 | doi:10.1038/nmeth.1264
A noncytotoxic DsRed variant for whole-cell labeling
Rita L Strack1,4, Daniel E Strongin2,4, Dibyendu Bhattacharyya2, Wen Tao3, Allison Berman2, Hal E Broxmeyer3, Robert J Keenan1 & Benjamin S Glick2
A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
- Department of Biochemistry and Molecular Biology, The University of Chicago, Gordon Center W238, Chicago, Illinois 60637, USA.
- Department of Molecular Genetics and Cell Biology, and Institute for Biophysical Dynamics, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA.
- Walther Oncology Center, Indiana University School of Medicine, 950 West Walnut Street, Room 302, Indianapolis, Indiana 46202, USA.
- These authors contributed equally to this work.
Correspondence to: Benjamin S Glick2 e-mail: bsglick@uchicago.edu
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