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Brief Communication
Nature Methods 4, 555–557 (1 July 2007) | doi:10.1038/nmeth1062
Bright monomeric red fluorescent protein with an extended fluorescence lifetime
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Abstract
Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability.
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