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Shown is a fluorescence image of mouse brain vasculature. Brain vasculature (cyan) can be genetically targeted with engineered adeno-associated viral capsid AAV-PHP. V1 here carries a fluorescent protein marker (magenta) after systemic injection into the mouse.
More basic research studies of marine microorganisms — supported by new methods, tools and resources — are needed to help inform policies to mitigate the impact of climate change.
“Every day sadder and sadder news of its increase. In the City died this week 7496; and of them, 6102 of the plague. But it is feared that the true number of the dead this week is near 10,000 ....” —Samuel Pepys, 1665
A resource of detailed DNA delivery and expression protocols for marine protists will enable new studies to understand the fundamental and ancestral features of eukaryotic cells.
The meltome atlas compiles the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans, providing a resource for analyzing protein stability in the context of function and interactions.
‘Nativeomics’ enables identification of ligands bound to membrane proteins through detection of intact protein–ligand assemblies followed by dissociation and identification of individual ligands within the same mass spectrometry experiment.
A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats.
KAS-seq applies N3-kethoxal to label guanines along single-stranded DNA in live cells, enabling characterization of ssDNA-involved transcription dynamics with as little as 1,000 cells.
Single-molecule displacement/diffusivity mapping (SMdM) enables nanoscale mapping of freely diffusing molecules in mammalian cells and reveals the structural basis of variations in local diffusivity in both the cytoplasm and nucleus.
In situ point spread function (PSF) retrieval (INSPR) enables precise single-molecule localization in 3D single-molecule localization microscopy of whole cells and tissues. It directly determines PSF from a single-molecule blinking dataset, removing errors associated with sample-induced aberrations.
M-CREATE is an in vivo screening strategy for identifying recombinant AAVs with desired tropism. The approach involves both positive and negative selection and yields vectors with diversified cell-type tropism that can cross the blood–brain barrier in adult mice across strains when delivered intravenously.