Volume 14 Issue 11, November 2017

How to run a computational competition energized by modern dance.

Fate maps of human brain cancer cells, transplanted in mouse, challenge traditional views of tumor genomic heterogeneity.

Ultralow concentrations of DNA can be optically sequenced with SMRT DNA sequencing.

Two new methods simultaneously measure epitope and transcriptome levels in single cells.

An optical setup for observing targeted fluorophores lasing from within tissue.

A method to analyze intact glycopeptides on a proteome-wide scale almost doubles the number of confirmed glycoproteins.

Antibodies that initiate signaling cascades and thereby replace reprogramming factors promise an efficient and safe way to produce pluripotent stem cells.

Image-guided targeting of fluorescently labeled neurons enables automated electrophysiological recordings of defined neuronal cell types.

A wealth of spatial techniques capture the 'where' of gene expression.

Efficient tracking and optical whole-brain imaging at single-cell resolution in freely behaving zebrafish larvae pave the way for quantitative investigation of circuits underlying complex behaviors.

Advances in image scanning microscopy move super-resolution imaging deeper into tissues with faster visualization and finer details.

This review outlines experimental considerations, advances and challenges in microbial single-cell genome sequencing and discusses the applications and scientific questions that this approach enabled.

This study compares naive human pluripotent stem cells, either reprogrammed directly from somatic cells or converted from primed cells, under a variety of culture conditions.

The Critical Assessment of Metagenome Interpretation (CAMI) community initiative presents results from its first challenge, a rigorous benchmarking of software for metagenome assembly, binning and taxonomic profiling.

MECAT combines fast alignment with error correction and de novo assembly for the processing of long single-molecule reads derived from large genomes.

Cryo-EM-based structure determination of macromolecular complexes at near-atomic resolution is possible using a mid-range 200-keV transmission electron microscope instrument.

NeuBtracker is an open-source platform that enables simultaneous imaging of behavior and neural activity in freely behaving zebrafish larvae. Its performance is demonstrated during several spontaneous or stimulus-induced behaviors.

SCENIC enables simultaneous regulatory network inference and robust cell clustering from single-cell RNA-seq data.

A rescanning microscopy approach enables two-photon image-scanning microscopy that doubles resolution relative to that of conventional two-photon microscopy at high frame rates and with high sensitivity for improved super-resolution imaging of living specimens.

A genetically encoded tension sensor module for measuring molecular forces at 3–5 pN along with tools for multiplexed tension sensing and data analysis reveal an intramolecular tension gradient across talin-1 during cell adhesion.

This paper reports the efficient generation of human alveolar cells from induced pluripotent stem cells and their expansion in 3D culture.

A tracking microscope for imaging neuronal activity at single-cell resolution in freely swimming zebrafish larvae is described. This technology allows functional imaging during behaviors that have previously been inaccessible to real-time analysis.