The use of photoswitchable fluorescent proteins in STED nanoscopy enables higher-contrast imaging with dramatically reduced photobleaching.
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References
Danzl, J.G. et al. Coordinate-targeted fluorescence nanoscopy with multiple off states. Nat. Photonics 10, 122–128 (2016).
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Strack, R. Protected STED nanoscopy. Nat Methods 13, 196–197 (2016). https://doi.org/10.1038/nmeth.3789
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DOI: https://doi.org/10.1038/nmeth.3789