Multicolor quantitative confocal imaging cytometry

Latest Research

  • Resource |

    This resource paper describes the steps involved in carrying out quantitative multicolour imaging in tissue. It is applied to cleared mouse bone and plots the spatial distribution of specific cell populations within the marrow.

    • Daniel L Coutu
    • , Konstantinos D Kokkaliaris
    • , Leo Kunz
    •  & Timm Schroeder
  • Brief Communication |

    RNA SPOTs adapts sequential fluorescence in situ hybridization (seqFISH) for highly accurate and inexpensive transcriptome-scale or targeted RNA quantification in vitro.

    • Chee-Huat Linus Eng
    • , Sheel Shah
    • , Julian Thomassie
    •  & Long Cai
  • Article |

    An improved MS2-tagging system for live-cell RNA imaging allows faithful monitoring of the mRNA life cycle, overcoming degradation artifacts associated with previous versions and having implications regarding mRNA regulation in yeast.

    • Evelina Tutucci
    • , Maria Vera
    • , Jeetayu Biswas
    • , Jennifer Garcia
    • , Roy Parker
    •  & Robert H Singer
  • Article |

    The high spatial resolution of secondary ion mass spectrometry and the high resolving power of the Orbitrap mass spectrometer are combined in a single imaging platform, the 3D OrbiSIMS. The instrument's capabilities for resolving lipids and neurotransmitters in the brain with subcellular spatial resolution, and a drug in a single cell in three dimensions is demonstrated.

    • Melissa K Passarelli
    • , Alexander Pirkl
    • , Rudolf Moellers
    • , Dmitry Grinfeld
    • , Felix Kollmer
    • , Rasmus Havelund
    • , Carla F Newman
    • , Peter S Marshall
    • , Henrik Arlinghaus
    • , Morgan R Alexander
    • , Andy West
    • , Stevan Horning
    • , Ewald Niehuis
    • , Alexander Makarov
    • , Colin T Dollery
    •  & Ian S Gilmore
  • Article |

    This paper describes methods for the 3D culture of mouse lung progenitor cells that can differentiate in vitro and in vivo along all epithelial lineages.

    • Massimo Nichane
    • , Asif Javed
    • , V Sivakamasundari
    • , Monisha Ganesan
    • , Lay Teng Ang
    • , Petra Kraus
    • , Thomas Lufkin
    • , Kyle M Loh
    •  & Bing Lim

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