Article abstract
Nature Immunology 8, 1217 - 1226 (2007)
Published online: 7 October 2007 | Corrected online: 19 October 2007 | doi:10.1038/ni1522
Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo
Shalin H Naik1,2, Priyanka Sathe1,3, Hae-Young Park1,4, Donald Metcalf1, Anna I Proietto1,3, Aleksander Dakic1, Sebastian Carotta1, Meredith O'Keeffe1,4, Melanie Bahlo1, Anthony Papenfuss1, Jong-Young Kwak1,4, Li Wu1 & Ken Shortman1
Abstract
The development of functionally specialized subtypes of dendritic cells (DCs) can be modeled through the culture of bone marrow with the ligand for the cytokine receptor Flt3. Such cultures produce DCs resembling spleen plasmacytoid DCs (pDCs), CD8+ conventional DCs (cDCs) and CD8- cDCs. Here we isolated two sequential DC-committed precursor cells from such cultures: dividing 'pro-DCs', which gave rise to transitional 'pre-DCs' en route to differentiating into the three distinct DC subtypes (pDCs, CD8+ cDCs and CD8- cDCs). We also isolated an in vivo equivalent of the DC-committed pro-DC precursor cell, which also gave rise to the three DC subtypes. Clonal analysis of the progeny of individual pro-DC precursors demonstrated that some pro-DC precursors gave rise to all three DC subtypes, some produced cDCs but not pDCs, and some were fully committed to a single DC subtype. Thus, commitment to particular DC subtypes begins mainly at this pro-DC stage.
- The Walter and Eliza Hall Institute, Parkville, Victoria 3050, Australia.
- The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands.
- Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, Australia.
- Present addresses: Department of Biochemistry, College of Medicine and Medical Research for Cancer Molecular Therapy, Dong-A University, Busan 602-714, Korea (H.-Y.P. and J.-Y.K.) and Bavarian Nordic, Munich 82152, Germany (M.O.).
Correspondence to: Shalin H Naik1,2 e-mail: s.naik@nki.nl
Correspondence to: Ken Shortman1 e-mail: shortman@wehi.edu.au
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