Nature Immunology 7, 868 - 874 (2006)
Published online: 9 July 2006; | doi:10.1038/ni1362
Detection of pathogenic intestinal bacteria by Toll-like receptor 5 on intestinal CD11c+ lamina propria cellsSatoshi Uematsu1, 7, Myoung Ho Jang2, 7, Nicolas Chevrier1, Zijin Guo2, Yutaro Kumagai1, Masahiro Yamamoto1, Hiroki Kato1, Nagako Sougawa2, Hidenori Matsui3, Hirotaka Kuwata4, Hiroaki Hemmi1, Cevayir Coban5, Taro Kawai6, Ken J Ishii6, Osamu Takeuchi1, 6, Masayuki Miyasaka2, Kiyoshi Takeda4
& Shizuo Akira1, 61
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565-0871, Japan. 2
Laboratory of Immunodynamics, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine (C8), 2-2, Yamada-oka, Suita, 565-0871, Japan. 3
Laboratory of Immunoregulation, Kitasato Institute for Life Sciences and Graduate School of Infection, Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan. 4
Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. 5
21st Century COE, Combined Program on Microbiology and Immunology, Osaka University, 3-1 Yamada-oka, Suita Osaka 565-0871, Japan. 6
ERATO, Japan Science and Technology Corporation, 3-1 Yamada-oka, Suita Osaka 565-0871, Japan. 7
These authors contributed equally to this work.
Correspondence should be addressed to Shizuo Akira sakira@biken.osaka-u.ac.jp Toll-like receptors (TLRs) recognize distinct microbial components and induce innate immune responses. TLR5 is triggered by bacterial flagellin. Here we generated Tlr5-/- 1mice and assessed TLR5 function in vivo. Unlike other TLRs, TLR5 was not expressed on conventional dendritic cells or macrophages. In contrast, TLR5 was expressed mainly on intestinal CD11c+ lamina propria cells (LPCs). CD11c+ LPCs detected pathogenic bacteria and secreted proinflammatory cytokines in a TLR5-dependent way. However, CD11c+ LPCs do not express TLR4 and did not secrete proinflammatory cytokines after exposure to a commensal bacterium. Notably, transport of pathogenic Salmonella typhimurium from the intestinal tract to mesenteric lymph nodes was impaired in Tlr5-/- mice. These data suggest that CD11c+ LPCs, via TLR5, detect and are used by pathogenic bacteria in the intestinal lumen.
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