Abstract
Loss-of-function mutations in the FLG (filaggrin) gene cause the semidominant keratinizing disorder ichthyosis vulgaris1 and convey major genetic risk for atopic dermatitis (eczema)2,3,4, eczema-associated asthma2,3 and other allergic phenotypes5. Several low-frequency FLG null alleles occur in Europeans and Asians, with a cumulative frequency of ∼9% in Europe4. Here we report a 1-bp deletion mutation, 5303delA, analogous to common human FLG mutations, within the murine Flg gene in the spontaneous mouse mutant flaky tail (ft). We demonstrate that topical application of allergen to mice homozygous for this mutation results in cutaneous inflammatory infiltrates and enhanced cutaneous allergen priming with development of allergen-specific antibody responses. These data validate flaky tail as a useful model of filaggrin deficiency and provide experimental evidence for the hypothesis that antigen transfer through a defective epidermal barrier is a key mechanism underlying elevated IgE sensitization and initiation of cutaneous inflammation in humans with filaggrin-related atopic disease.
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Acknowledgements
We thank B. Mistry, A. Smyth, T. Adachi and M. Furuhashi for their technical assistance and T. Yoshida for his cooperation. We are grateful for the assistance of B. Cloak with photomicrography and S. Worrell with histopathology. This work was supported by the Labour Sciences Research Grants for Research on Allergic Disease and Immunology from the Ministry of Health, Labour, and Welfare of Japan (M.A.), National Institute of Health grants P01 AM21557 (P.F.), R01 AR49183 (R.B.P.) and the Odland Endowed Research Fund (P.F.). P.G.F. was supported by Science Foundation Ireland. A.D.I. is supported by the Children's Medical and Research Foundation, OLCHC. Filaggrin research in the McLean laboratory is supported by grants from The British Skin Foundation, The National Eczema Society, The Medical Research Council (Reference number G0700314) and donations from anonymous families affected by eczema in the Tayside Region of Scotland.
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The study was designed by W.H.I.M., P.G.F., A.D.I and M.A. Molecular biology was performed by T.S., A. Sandilands, L.E.C., H.K., A. Shiohama, A.K., N.S. and J.K. Bioinformatics was performed by W.H.I.M. C-terminal profilaggrin antibody and immunoblot data was generated by R.B.P. and P.F. The initial mixed strain ft mice were provided by J.P.S. Mouse backcrossing, immunology and histological experiments were performed by P.G.F., S.P.S., N.E.M and J.J.C. The manuscript was written by P.G.F., T.S., J.K., M.A., R.B.P., A.D.I. and W.H.I.M.
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Fallon, P., Sasaki, T., Sandilands, A. et al. A homozygous frameshift mutation in the mouse Flg gene facilitates enhanced percutaneous allergen priming. Nat Genet 41, 602–608 (2009). https://doi.org/10.1038/ng.358
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DOI: https://doi.org/10.1038/ng.358
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