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Letter

Nature Genetics 33, 401–406 (1 March 2003) | doi:10.1038/ng1117

A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference

Douglas A. Rubinson , Christopher P. Dillon , Adam V. Kwiatkowski , Claudia Sievers , Lili Yang , Johnny Kopinja , Mingdi Zhang , Michael T. McManus , Frank B. Gertler , Martin L. Scott & Luk Van Parijs

RNA interference (RNAi) has recently emerged as a specific and efficient method to silence gene expression in mammalian cells either by transfection of short interfering RNAs (siRNAs; ref. 1) or, more recently, by transcription of short hairpin RNAs (shRNAs) from expression vectors and retroviruses.