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Existing methods for detecting DNA methylation (5mC) are less accurate and robust. Here, the authors develop a deep learning tool ccsmeth and a Nextflow pipeline ccsmethphase for genome-wide 5mCpG detection and phasing with high accuracy from CCS reads in human.
Twisted bilayer graphene hosts a zoo of rich correlated electronic phases. Here, the authors explore the phase diagram of a twisted bilayer graphene/tungsten diselenide heterostructure and uncover a series of delicate Fermi surface reconstructions and signatures of orbital magnetism.
E217 is a Myoviridae used in an experimental phage cocktail to eradicate Pseudomonas aeruginosa. Here, the authors utilize cryo-EM and functional analysis to delineate E217 structural proteins, tail dynamics, and mechanisms of host recognition.
Traditional type 2 diabetes (T2D) screening methods often depend on age, BMI guidelines and glucose measurements. Here, authors use a deep learning model that leverages chest radiographs and electronic health record data to screen for T2D, highlighting potential for early detection and intervention.
Achieving stable lithium metal anodes under large current densities over 10 mA cm−2 and area capacities over 10 mAh cm−2 remains a critical challenge. Here, authors propose a dynamic gel as protect layer for lithium metal anode to facilitate with the Young’s modulus, flexibility and ionic conductivity, thus to stabilize the lithium metal anode.
The exact function of kinetochore proteins in meiosis remains unclear. Using live imaging of C. elegans oocytes, the authors systematically study the contribution of each kinetochore sub-complex and describe a push-pull mechanism that confers robustness to chromosome segregation.
The mechanism of action of the antibacterial tripeptide AMC-109 is unclear. Here, Melcrová et al. show that AMC-109 self-assembles into stable aggregates with a cationic surface, and then individual peptides insert into the bacterial membrane and disrupt membrane nanodomains, thus affecting membrane function without forming pores.
Efficient delivery of extracellular vesicles to the injured spinal cord, with minimal damage, remains challenging. Here, the authors fabricate a minimally invasive microneedle device, which provides efficient and sustained extracellular vesicle delivery for spinal cord injury treatment.
Adolescent drug use augments psychiatric risk. Here the authors show that abused drugs dysregulate adolescent Netrin-1/DCC signaling, triggering ectopic long-distance dopamine axon growth in males while Netrin1 compensatory events protect females.
Bacterial resistance to sulfonamide antibiotics (sulfas) is mediated by acquisition of sul genes, which encode sulfa-insensitive versions of the target enzyme, dihydropteroate synthase. Here, Venkatesan et al. study Sul enzymes using biochemical, structural, mutational and functional analyses, revealing the molecular basis for Sul-mediated drug resistance.
Endothelial cell CD36 controls tissue fatty acid uptake. Here the authors show how fatty acid uptake by endothelial cells involves regulation of membrane ceramide production, caveolae dynamics, and exosome generation, these events facilitate transfer of circulating fatty acids to tissues and communication between endothelium and parenchyma.
Aromatase inhibitors are an effective treatment for ER+ breast cancer, but response is variable. Here, the authors undertake molecular analyse of response from the POETIC trial and identify several factors associated with response.
Natural and engineered Poisson’s ratios obey fundamental bounds determined by stability, linearity, and thermodynamics. Here, the authors design and realize self-bridging metamaterials with Poisson’s ratios surpassing the theoretical bounds.
Changes in the abundance and diversity of neural cell types provide the substrate for behavioral evolution. This study provides evidence of extensive, mosaic expansion of an integration brain center, among closely related Heliconiini butterflies, associated with increased neuron number, visual processing and long term memory.
Level of atomic disorder in materials is critical to understanding the effect of local structure on materials properties. Here the authors present a workflow combining structure-aware graph neural networks and physics-inspired order parameter to characterize structural disorder on a per atom basis.
IgG3 antibodies have potent effector functions, but are not used as therapeutics and structural data are missing. Here, the authors combine cryoEM and MS to study IgG3-mediated complement activation to provide the first structural insights into IgG3.
The existence of peripheric charged groups on poly-N-isopropylacrylamide (pNIPAM) microgels together with the corresponding counter ion clouds has been proposed to be responsible for the spontaneous deswelling of pNIPAM microgels in concentrated suspensions but no direct measurement of such an ionic cloud exists. Here, the authors use small-angle neutron scattering with contrast variation with different ions to isolate the change in the form factor directly related to the counterion cloud and obtain its radius and width.
Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Here, the authors propose a spatial histopathology tool that allows for high-plex protein staining and physical expansion, while retaining the lateral tissue expansion.
In this work the authors investigate two types of secretins in Escherichia coli, GspDα and GspDβ, and report the Cryo-ET in situ structures of their key intermediate states of during the translocation process. Yielding a resolution ranging from 9 Å to 19 Å, the structures allow the identification of different membrane interaction patterns and ways of transitioning the peptidoglycan layer. These results suggest two distinct models for the membrane translocation of GspDα and GspDβ and provide insights into the inner to outer membrane biogenesis of T2SS secretins.
Laser-produced plasma can be used for particle acceleration in different schemes. Here the authors demonstrate proton acceleration from the intense ultrashort laser pulse interaction with micron-sized cryogenic hydrogen jet.