Directed evolution of nucleic acids and proteins in the laboratory can produce many novel phenotypes in a relatively short time frame. A significant enhancement to these techniques allows continuous evolution in special turbidostats and can speed the discovery of proteins with desirable activities.
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References
Joyce, G.F. Angew. Chem. Int. Edn Engl. 46, 6420–6436 (2007).
Esvelt, K.M., Carlson, J.C. & Liu, D.R. Nature published online, 10.1038/nature09929 (10 April 2011).
Wright, M.C. & Joyce, G.F. Science 276, 614–617 (1997).
Paegel, B.M., Joyce, G.F. & Wickens, M. PLoS Biol. 6, e85 (2008).
Voytek, S.B. & Joyce, G.F. Proc. Natl. Acad. Sci. USA 104, 15288–15293 (2007).
Díaz Arenas, C. & Lehman, N. The continuous evolution in vitro technique. in Current Protocols in Nucleic Acid Chemistry (ed. Herdewijn, P.) Ch. 9, 9.7.1–9.1.17 (John Wiley & Sons, 2010).
Arnold, F.H. & Georiou, G. (eds.). Directed Enzyme Evolution: Screening and Selection Methods. Vol. 230 (Humana Press, 2003).
Smith, G.P. & Petrenko, V.A. Chem. Rev. 97, 391–410 (1997).
Eigen, M. Steps Towards Life: A Perspective on Evolution. (Oxford University Press, 1992).
Iwata-Reuyl, D. Curr. Opin. Chem. Biol. 12, 126–133 (2008).
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Lehman, N. Protein evolution at warp speed. Nat Chem Biol 7, 252–253 (2011). https://doi.org/10.1038/nchembio.568
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DOI: https://doi.org/10.1038/nchembio.568