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Brief Communication

Nature Chemical Biology 5, 23–25 (1 January 2009) | doi:10.1038/nchembio.126

Peptidase substrates via global peptide profiling

Debarati M Tagore , Whitney M Nolte , John M Neveu , Roberto Rangel , Liliana Guzman-Rojas , Renata Pasqualini , Wadih Arap , William S Lane & Alan Saghatelian

Peptide metabolism is a complex process that involves many proteins working in concert. Mass spectrometry–based global peptide profiling of mice lacking dipeptidyl peptidase 4 (DPP4) identified endogenous DPP4 substrates and revealed an unrecognized pathway during proline peptide catabolism that interlinks aminopeptidase and DPP4 activities.