Scheme 1 - QUIC-Tag strategy for quantitative O-GlcNAc proteomics.

From the following article

Probing the dynamics of O-GlcNAc glycosylation in the brain using quantitative proteomics

Nelly Khidekel, Scott B Ficarro, Peter M Clark, Marian C Bryan, Danielle L Swaney, Jessica E Rexach, Yi E Sun, Joshua J Coon, Eric C Peters & Linda C Hsieh-Wilson

Nature Chemical Biology 3, 339 - 348 (2007) Published online: 13 May 2007

doi:10.1038/nchembio881

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(a) O-GlcNAc-glycosylated proteins are chemoenzymatically tagged with a ketogalactose sugar, which allows selective biotinylation of the proteins. (b) O-GlcNAc proteins from two different cell states are selectively tagged, proteolyzed and differentially labeled with 'light' or 'heavy' isotopes. The mixtures are combined, and O-GlcNAc peptides of interest are specifically enriched by avidin chromatography for selective quantification by LC-MS.

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