Genetic manipulation of biosynthetic pathways is a useful method for producing analogs of complex bioactive metabolites, but this technique can be challenging when performed in the natural producer of the target compounds. Reconstruction of biosynthetic gene clusters in E. coli could be the key to rapid heterologous production of natural products and genetic manipulation of their biosynthetic pathways.
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Katie Ris
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Challis, G. Engineering Escherichia coli to produce nonribosomal peptide antibiotics. Nat Chem Biol 2, 398–400 (2006). https://doi.org/10.1038/nchembio0806-398
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DOI: https://doi.org/10.1038/nchembio0806-398
