Abstract
Ozone exposure is a growing global health problem, especially in urban areas. While ozone in the stratosphere protects the earth from harmful ultraviolet light, tropospheric or ground-level ozone is toxic and can damage the respiratory tract. It has recently been shown that ozone may be produced endogenously in inflammation and antibacterial responses of the immune system; however, these results have sparked controversy owing to the use of a non-specific colorimetric probe. Here we report the synthesis of fluorescent molecular probes able to unambiguously detect ozone in both biological and atmospheric samples. Unlike other ozone-detection methods, in which interference from different reactive oxygen species is often a problem, these probes are ozone specific. Such probes will prove useful for the study of ozone in environmental science and biology, and so possibly provide some insight into the role of ozone in cells.
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Change history
10 March 2010
In the version of this Article originally published, some of the images for the different parts in Fig. 4 were incorrectly placed. The correction has been made in the HTML and PDF versions of the Article. A Supplementary film was also missing online, this has now been uploaded.
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Acknowledgements
We acknowledge support from the US National Science Foundation (to K.K.) and the US National Institutes of Health (to C.M.S., B.R.P. and G.D.L). We thank Yang Gao for determining the specificity of compound 8 for ozone.
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A.L.G., C.M.S., B.R.P, G.D.L. and K.K. designed the experiments. A.L.G. generated the data shown in Figs 2, 3 and 5. C.M.S. generated the data shown in Fig. 4. S.A. synthesized and characterized compound 8. A.L.G., C.M.S. and K.K. wrote the manuscript.
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Garner, A., St Croix, C., Pitt, B. et al. Specific fluorogenic probes for ozone in biological and atmospheric samples. Nature Chem 1, 316–321 (2009). https://doi.org/10.1038/nchem.240
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DOI: https://doi.org/10.1038/nchem.240
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