Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Phospho-specific antibodies by design

Nature Biotechnology volume 31, pages 889–891 (2013)Cite this article

Subjects

Antibodies specific to phosphorylated peptides are readily generated through structure-based engineering and in vitro selection.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Strategy of Koerber et al.1 for generating phospho- and target-specific antibodies.

References

  1. Koerber, J.T., Thomsen, N.D., Hannigan B.T., Degrado, W.F. & Wells J.A., Nat. Biotechnol. 31, 916–921 (2013).

    Article  CAS  Google Scholar 

  2. Hunter, T. Curr. Opin. Cell Biol. 21, 140–146 (2009).

    Article  CAS  Google Scholar 

  3. Olsen, J.V. et al. Cell 127, 635–648 (2006).

    Article  CAS  Google Scholar 

  4. Brumbaugh, K. et al. Methods Mol. Biol. 717, 3–43 (2011).

    Article  CAS  Google Scholar 

  5. Shih, H. et al. J. Biol. Chem. 287, 44425–44434 (2012).

    Article  CAS  Google Scholar 

  6. Bradbury, A.R., Sidhu, S., Dübel, S. & McCafferty, J. Nat. Biotechnol. 29, 245–254 (2011).

    Article  CAS  Google Scholar 

  7. Marx, V. Nat. Methods 10, 829–833 (2013).

    Article  CAS  Google Scholar 

  8. Stoevesandt, O. & Taussig, M.J. New Biotechnol. 29, 511–514 (2012).

    Article  CAS  Google Scholar 

  9. Uhlén, M. et al. Nat. Biotechnol. 28, 1248–1250 (2010).

    Article  Google Scholar 

  10. Hornbeck, P.V. et al. Nucleic Acids Res. 40 Database issue, D261–D270 (2012).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Oda Stoevesandt and Michael J. Taussig are at the Protein Technology Group, The Babraham Institute, Cambridge, UK.,

    Oda Stoevesandt & Michael J Taussig

Authors
  1. Oda Stoevesandt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael J Taussig
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Oda Stoevesandt or Michael J Taussig.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stoevesandt, O., Taussig, M. Phospho-specific antibodies by design. Nat Biotechnol 31, 889–891 (2013). https://doi.org/10.1038/nbt.2712

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.2712

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing