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High Efficiency Direct Gene Transfer to Plants

Bio/Technology volume 3, pages 1099–1103 (1985)Cite this article

Abstract

The efficiency of direct gene transfer to plant protoplasts has been increased more than 1000 fold over that previously reported. On the order of 2 percent of all colonies recovered without selection were transformed. The improvement was due to: treatment of protoplasts with a high voltage electric pulse (electroporation), optimization of the polyethylene glycol (PEG) concentration, addition of PEG after the DNA and the application of a heat shock (5 minutes at 45°C). These factors are of equal importance in achieving high transformation efficiencies.

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Authors and Affiliations

  1. Friedrich Miescher Institute, P.O. Box 2543, CH-4002, Basel, Switzerland

    R. D. Shillito, M. W. Saul, J. Paszkowski, M. Müller & I. Potrykus

Authors
  1. R. D. Shillito
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  2. M. W. Saul
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  3. J. Paszkowski
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  4. M. Müller
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  5. I. Potrykus
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Shillito, R., Saul, M., Paszkowski, J. et al. High Efficiency Direct Gene Transfer to Plants. Nat Biotechnol 3, 1099–1103 (1985). https://doi.org/10.1038/nbt1285-1099

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  • DOI: https://doi.org/10.1038/nbt1285-1099

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