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Research
Nature Biotechnology 23, 736–740 (1 June 2005) | doi:10.1038/nbt1097
Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies
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Abstract
Segmental isotopic labeling of proteins using protein ligation is a recently established in vitro method for incorporating isotopes into one domain or region of a protein to reduce the complexity of NMR spectra, thereby facilitating the NMR analysis of larger proteins. Here we demonstrate that segmental isotopic labeling of proteins can be conveniently achieved in Escherichia coli using intein-based protein ligation.
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