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Article
Nature Biotechnology  21, 275 - 280 (2003)
Published online: 18 February 2003; | doi:10.1038/nbt796


There is a Corrigenda (April 2003) associated with this Article.

Human zinc fingers as building blocks in the construction of artificial transcription factors

Kwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1, Moon-Sun Hwang1, Eun-Hyun Ryu1, Kyung-Soon Park1, Hyo-Young Yang1, Dong-ki Lee1, Yangsoon Lee1, Jinwoo Park1, Heung Sun Kwon1, Hyun-Won Kim3, Byung-Il Yeh3, Hyean-Woo Lee3, Soon Hyung Sohn3, Joonho Yoon3, Wongi Seol1 & Jin-Soo Kim1

1  ToolGen Inc., 461-6 Jeonmin-Dong, Yusung-Gu, Daejeon, 305-390, South Korea.

2  School of Biological Sciences, Seoul National University, Seoul, 151-742, South Korea.

3  Department of Biochemistry and Institute of Basic Medical Sciences and IFBB, Yonsei University, Wonju College of Medicine, Wonju, 220-701, South Korea.

4  These authors contributed equally to this work.

Correspondence should be addressed to Jin-Soo Kim jsk@toolgen.com
We describe methods for generating artificial transcription factors capable of up- or downregulating the expression of genes whose promoter regions contain the target DNA sequences. To accomplish this, we screened zinc fingers derived from sequences in the human genome and isolated 56 zinc fingers with diverse DNA-binding specificities. We used these zinc fingers as modular building blocks in the construction of novel, sequence-specific DNA-binding proteins. Fusion of these zinc-finger proteins with either a transcriptional activation or repression domain yielded potent transcriptional activators or repressors, respectively. These results show that the human genome encodes zinc fingers with diverse DNA-binding specificities and that these domains can be used to design sequence-specific DNA-binding proteins and artificial transcription factors.

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Nature Biotechnology
ISSN: 1087-0156
EISSN: 1546-1696
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