 | Figure 3
Nature Biotechnology
19, 769 - 772 (2001)
doi:10.1038/90831
Direct visualization of protein interactions in plant cellsRajagopal Subramaniam, Darrell Desveaux, Catherine Spickler, Stephen W. Michnick
& Normand Brisson | | | | Figure 3. The SA-induced interaction between NPR1/NIM1 and TGA2 is located in the nucleus.
(A−F) Potato protoplasts electroporated with NPR1/NIM1−DHFR-F[1,2] and TGA2−DHFR-F[3] were examined by fluorescence microscopy. Individual protoplasts (A−D) and a group of protoplasts (E, F) were visualized using a fluorescein filter (excitation 460  10 nm and emission 520 10 nm) to detect fluorescence due to fMTX (A, C, E) or a UV filter (excitation 365 nm and emission 420 nm) to detect DAPI staining (B, D, F). (A, B) protoplasts not treated with SA. (C−F) Protoplasts treated with 700  M SA. Arrows in E and F indicate nuclei. On average, 60% of electroporated protoplasts treated with SA showed nuclear localized fluorescence. Magnification: A−D,  100; E, F, 40.
|
| | | |  |
|
