FIGURE 2. TLR9 exits the ER and travels through the Golgi before cleavage.
From the following article:
The ectodomain of Toll-like receptor 9 is cleaved to generate a functional receptor
Sarah E. Ewald, Bettina L. Lee, Laura Lau, Katherine E. Wickliffe, Guo-Ping Shi, Harold A. Chapman & Gregory M. Barton
Nature 456, 658-662(4 December 2008)
doi:10.1038/nature07405
a, Cleaved TLR9 is EndoH-resistant. Proteins from lysates or purified phagosomes from RAW-TLR9 cells were immunoprecipitated with anti-HA resin and treated with EndoH (E), PNGaseF (P), or left untreated (-). b, A longer exposure of TLR9 immunoprecipitated from RAW-TLR9 cells and subjected to analysis as described in a. An asterisk indicates the high-molecular-mass full-length TLR9 band that is EndoH-resistant. c, TLR9 cleavage requires passage through the Golgi. Pulse–chase analysis was performed, at the indicated chase time points, on RAW-TLR9 cells treated with brefeldin A (B) or vehicle (-). The data presented in each panel are representative of at least three experiments.
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