Supplementary information

From the following article:

Kruppel-like factor 2 regulates thymocyte and T-cell migration

Corey M. Carlson, Bart T. Endrizzi, Jinghai Wu, Xiaojie Ding, Michael A. Weinreich, Elizabeth R. Walsh, Maqsood A. Wani, Jerry B. Lingrel, Kristin A. Hogquist and Stephen C. Jameson

Nature 442, 299-302(20 July 2006)

doi:10.1038/nature04882

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Supplementary Notes

This file contains Supplementary Methods with eight references, details of grant support and Supplementary Figures S1–S4 with legends. Supplementary Figure S1 details the normal in vitro survival of KLF2-/- mature thymocytes. Supplementary Figure S2 shows aberrant trafficking and phenotype of KLF2-/- SP thymocytes after adoptive transfer. Supplementary Figure S3 details real-time PCR analysis of gene expression in KLF2-/- and KLF2+/- DP and CD4 SP thymocytes. Supplementary Figure S4 shows the sequence of human and mouse S1P1 promoters, showing the CACCC sequence and the location of primers used for the CHiP assay.

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