Supplementary information

From the following article:

Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres

Young Chung, Irina Klimanskaya, Sandy Becker, Joel Marh, Shi-Jiang Lu, Julie Johnson, Lorraine Meisner and Robert Lanza

Nature 439, 216-219(12 January 2006)

doi:10.1038/nature04277

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Supplementary Figure 1

PCR analysis of LacZ and GFP genes in ES and TS cell lines.

Supplementary Figure 2

LacZ stained placenta of d 10.5 chimera showing contribution of single blastomere-derived TS cells.

Supplementary Figure 3

PCR analysis demonstrating the presence of the LacZ gene in purified sperm from chimeric mice.

Supplementary Table S1

Derivation and Characterization of ES Cell lines from Single Blastomeres.

Supplementary Figure Legends

Text to accompany the above Supplementary Figures.

Supplementary Methods

Details of the Genomic DNA polymerase chain reaction.

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