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Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication

Nature volume 341, pages 503–507 (1989)Cite this article

Abstract

Simian virus 40 large tumour antigen (T) is a replication origin binding protein required for viral DNA synthesis. Unphosphorylated T antigen is deficient in promoting DNA replication in vitro but can be activated by phosphorylation at residue threonine 124 by the cdc2 protein kinase. This observation demonstrates that T is regulated by phosphorylation and provides a model for cdc2 function in the control of DNA replication.

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Author information

Author notes

  1. Ian Mohr

    Present address: Department of Biochemistry, University of California, Berkeley, California, 94720, USA

  2. Yasha Gluzman: Lederle Laboratories, Perl River, New York 10965, USA

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, P.O. Box Box 100, Cold Spring Harbor, New York, 11724, USA

    Duncan McVey, Leonardo Brizuela, Ian Mohr, Daniel R. Marshak, Yasha Gluzman & David Beach

Authors
  1. Duncan McVey
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  2. Leonardo Brizuela
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  3. Ian Mohr
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  4. Daniel R. Marshak
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  5. Yasha Gluzman
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  6. David Beach
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McVey, D., Brizuela, L., Mohr, I. et al. Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication. Nature 341, 503–507 (1989). https://doi.org/10.1038/341503a0

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