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A new human immunoglobulin VH family preferentially rearranged in immature B-cell tumours

Nature volume 331, pages 446–449 (1988)Cite this article

Abstract

The prevalent forms of adult and childhood B-cell neoplasia are chronic lymphocytic (CLL) and acute lymphocytic (ALL) leukaemia, and are typified by a nearly monoclonal accumulation of cells expressing a single heavy (H) and light (L) chain variable (V) region. V gene selection could be random, or quite biased if the disease or the developmental status of the transformed cell somehow influenced DNA rearrangement. We have cloned and sequenced three germ-line VH gene segments that constitute a new human VH family (subgroup V) linked within 160 kilobase pairs of the DH-JH complex. One VH(V) member is rearranged in about 30% of patients with CLL and ALL, but not in IgM-expressing B-cell lines from peripheral blood. In some tumours, we detect a truncated ( VH(V) RNA devoid of constant regions that originates from unrearranged VH(V) genes. In other tumours and in resting splenocytes, we detect large amounts of normally sized VH(V)-associated mRNA, although stimulation by mitogen of splenic B cells results in loss of VH(V)-hybridizing RNA. These features suggest that biased rearrangement of subgroup V may be under developmental selection.

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Author notes

  1. A. Shen and F. R. Blattner: Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA

Authors and Affiliations

  1. Department of Microbiology, University of Texas Health Science Center, Dallas, Texas, 75235, USA

    C. G. Humphries, A. Shen, W. A. Kuziel, J. D. Capra, F. R. Blattner & P. W. Tucker

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  1. C. G. Humphries
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  2. A. Shen
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  3. W. A. Kuziel
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  4. J. D. Capra
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  5. F. R. Blattner
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  6. P. W. Tucker
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Humphries, C., Shen, A., Kuziel, W. et al. A new human immunoglobulin VH family preferentially rearranged in immature B-cell tumours. Nature 331, 446–449 (1988). https://doi.org/10.1038/331446a0

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