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Expression of two types of E2A-HLF fusion proteins in YCUB-2, a novel cell line established from B-lineage leukemia with t(17;19)

Leukemia volume 15, pages 995–997 (2001)Cite this article

The t(17;19)(q21-q22;p13) is a recurring chromosomal translocation that is rarely found in B-lineage acute lymphoblastic leukemia (ALL) of childhood.1 This translocation results in the in frame fusion of the 5′-region of the E2A gene on chromosome 19 and the 3′-region of the HLF gene on chromosome 17. Its product, E2A-HLF chimeric protein, is responsible for the pathogenesis of t(17;19)-ALL in that it inhibits lymphoid precursor cells from undergoing apoptosis.2,3 This paper reports the establishment of a new B-lineage cell line with t(17;19), YCUB-2, which expresses two different types of E2A-HLF fusion proteins.

This cell line, established in 1997, was derived from a peripheral blood sample taken from a 4-year-old Japanese boy with ALL carrying the t(17;19) chromosomal abnormality. The leukemic cells presented the surface markers CD10, CD19, CD20, CD33, and HLA-DR. Chromosomal analysis showed 46,XY,t(17;19)(q22;p13.3). The patient suffered from hypercalcemia due to overproduction of parathyroid hormone-related protein in the leukemic cells. There was no evidence of disseminated intravascular coagulation (DIC). Although complete remission was achieved with chemotherapy, he relapsed 5 months later and died of progressive disease. The leukemic cells isolated from a peripheral blood sample obtained at the time of refractory disease, were cultured in RPMI1640 medium supplemented with 10% fetal calf serum for over 12 months, and the established cell line, YCUB-2, was isolated and characterized.

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  1. Department of Pediatrics, Yokohama City University School of Medicine, Yokohama, Japan

    H Takahashi, H Goto, T Funabiki, H Fujii, S Yamazaki, K Fujioka, K Ikuta & S Kai

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  1. H Takahashi
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  2. H Goto
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  3. T Funabiki
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Takahashi, H., Goto, H., Funabiki, T. et al. Expression of two types of E2A-HLF fusion proteins in YCUB-2, a novel cell line established from B-lineage leukemia with t(17;19). Leukemia 15, 995–997 (2001). https://doi.org/10.1038/sj.leu.2402120

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