George F Murphy

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Advancing the understanding of human and experimental disease

Latest Research

  • Article
    | Open Access

    RNA of sufficient quality and quantity can be extracted from formalin-fixed paraffin-embedded (FFPE) samples to obtain comprehensive transcriptome profiling using the 3′ massive analysis of c-DNA ends (MACE) RNA-sequencing technology. Thus, MACE provides an opportunity for utilizing FFPE samples stored in histological archives.

    • Stefaniya Boneva
    • , Anja Schlecht
    • , Daniel Böhringer
    • , Hans Mittelviefhaus
    • , Thomas Reinhard
    • , Hansjürgen Agostini
    • , Claudia Auw-Haedrich
    • , Günther Schlunck
    • , Julian Wolf
    •  & Clemens Lange
  • Article |

    This study reveals that inhibition of miR-181a-5p protects immature rats from epilepsy, including hippocampal insults, neuronal apoptosis, astrocyte and microglia activation, neuroinflammation, and oxidative stress. This protection is achieved through the SIRT1 pathway, which may be a novel therapeutic target for epilepsy.

    • Huimin Kong
    • , Huaili Wang
    • , Zhihong Zhuo
    • , Zhenbiao Li
    • , Peichao Tian
    • , Jing Wu
    • , Jian Liu
    • , Zheng Chen
    • , Jiyao Zhang
    •  & Qiang Luo
  • Article |

    This study provides insight into the function of Sox13 in hepatocellular carcinoma (HCC). Sox13 serves as a key regulator of HCC cell migration, invasion, and epithelial-to-mesenchymal transition by transactivating Twist1. Sox13 forms heterodimers with Sox5 to enhance Twist1 transcriptional activity. In addition, overexpression of Sox13 indicates poor prognosis for HCC patients. These findings implicate Sox13 as a potential therapeutic target for HCC.

    • Min Feng
    • , Fei Fang
    • , Ting Fang
    • , Hui Jiao
    • , Song You
    • , Xiaomin Wang
    •  & Wenxiu Zhao
  • Article
    | Open Access

    The authors. show that CCL3 derived from both tumor-associated macrophages and esophageal squamous cell carcinoma (ESCC) cells promotes cell migration and invasion of ESCC cells via binding CCR5. High expression of CCL3 and/or CCR5 associates with poor prognosis in ESCC patients. CCL3–CCR5 axis could be a specific target of anti-cancer therapy.

    • Takayuki Kodama
    • , Yu-ichiro Koma
    • , Noriaki Arai
    • , Aya Kido
    • , Naoki Urakawa
    • , Mari Nishio
    • , Manabu Shigeoka
    •  & Hiroshi Yokozaki
  • Article |

    Medulloblastoma (MB) cells form three-dimensional tumoroids in vitro that can be passaged and preserved. MB cells in tumoroids retain the tumorigenic potential and hedgehog signaling, which relies on stromal astrocytes and astrocyte-derived extracellular matrix. These findings provide a valuable cell model for the basic and preclinical studies of MB.

    • Yan Cheng
    • , Janusz Franco-Barraza
    • , Yuan Wang
    • , Chaonan Zheng
    • , Li Zhang
    • , Yanghui Qu
    • , Yaqiu Long
    • , Edna Cukierman
    •  & Zeng-jie Yang
  • Article |

    Intestinal farnesoid X receptor (FXR) deficiency in mice leads to more severe liver injury and inflammation with ethanol treatment, which is associated with increased intestinal leakage. These results suggest that intestinal FXR may be critical in maintaining gut integrity and the epithelial barrier to protect the liver from ethanol-induced injury.

    • Mingxing Huang
    • , Bo Kong
    • , Min Zhang
    • , Daniel Rizzolo
    • , Laura E. Armstrong
    • , Justin D. Schumacher
    • , Monica D. Chow
    • , Yi-Horng Lee
    • , Laurie B. Joseph
    • , Mary Stofan
    • , Lanjing Zhang
    •  & Grace L. Guo

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United States & Canadian Academy of Pathology 109th Annual Meeting Abstracts: Eyes on You

“Security Retina Scanner on Blue Human Eye” Geebshot/iStock

United States & Canadian Academy of Pathology 109th Annual Meeting Abstracts: Eyes on You

The largest gathering of physician-pathologists in the world