George F Murphy

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Advancing the understanding of human and experimental disease

Latest Research

  • Article |

    Digital spatial profiling is a new high-plex technology with potential to multiplex hundreds of proteins on a single slide. Here the authors validate the digital aspect of the technology on a control tissue microarray with known amounts of PD-L1 expression to show it has quantitative capacity comparable to quantitative immunofluorescence.

    • Swati Gupta
    • , Jon Zugazagoitia
    • , Sandra Martinez-Morilla
    • , Kit Fuhrman
    •  & David L. Rimm
  • Article |

    The interaction of genetic and epigenetic mechanisms is one of the underlying causes of phenotypic variability in diseases like type 2 diabetes. The authors identified the methylation-dependent CpG-SNP in the MTHFD1 gene as a regulator of allele-specific gene expression in peripheral blood mononuclear cells, which may perturb one-carbon metabolism in diabetes.

    • Manik Vohra
    • , Prabha Adhikari
    • , Sydney C.D’. Souza
    • , Shivashankar K. Nagri
    • , Shashikiran Umakanth
    • , Kapaettu Satyamoorthy
    •  & Padmalatha S. Rai
  • Article |

    The authors observed an increase of talin-vinculin spatial proximities in the livers of spotted fever Rickettsia australis or Ebola virus-infected mice when compared to mock mice. Furthermore, using EPAC1 knockout mice, they found that deletion of EPAC1 suppresses the formation of spatial proximal complex of talin-vinculin in rickettsial infections.

    • Yakun Liu
    • , Jie Xiao
    • , Ben Zhang
    • , Thomas R. Shelite
    • , Zhengchen Su
    • , Qing Chang
    • , Barbara Judy
    • , Xiang Li
    • , Aleksandra Drelich
    • , Jiani Bei
    • , Yixuan Zhou
    • , Junying Zheng
    • , Yang Jin
    • , Shannan L. Rossi
    • , Shao-Jun Tang
    • , Maki Wakamiya
    • , Tais Saito
    • , Thomas Ksiazek
    • , Bhupendra Kaphalia
    •  & Bin Gong
  • Article |

    Synovial mesenchymal stem cells (MSCs) are an attractive cell source for transplantation in the meniscus of the knee because of their high chondrogenic potential. This study describes the morphological features of synovial MSC adhesion to the meniscus and provides ways to increase the number of adherent MSCs so that clinical outcomes can be improved.

    • So Suzuki
    • , Mitsuru Mizuno
    • , Yuriko Sakamaki
    • , Ayako Mimata
    • , Kentaro Endo
    • , Yuji Kohno
    • , Nobutake Ozeki
    • , Koji Otabe
    • , Hisako Katano
    • , Kunikazu Tsuji
    • , Hideyuki Koga
    •  & Ichiro Sekiya
  • Article |

    SiO2-mediated epithelial–mesenchymal transition via the nuclear transcription factor Ets-like protein-1 (ELK-1) due to oxidative stress and the downstream signaling of ZC3H4 expression. These findings suggest that the nuclear transcription factor ELK-1 may be useful as a novel target for the treatment of pulmonary fibrosis.

    • Rong Jiang
    • , Qianqian Gao
    • , Mingxia Chen
    •  & Tingting Yu
  • Article |

    The ability to characterize the cellular composition and spatial organization of the tumor microenvironment has been limited by the techniques available to image the necessary number of biomarkers for broad phenotyping at a subcellular resolution. This study demonstrates the capabilities of Multiplexed Ion Beam Imaging (MIBI) for cell phenotype identification and their spatial relationships across multiple tumor types.

    • Jason Ptacek
    • , Darren Locke
    • , Rachel Finck
    • , Mary-Ellen Cvijic
    • , Zhuyin Li
    • , Jay G. Tarolli
    • , Murat Aksoy
    • , Yari Sigal
    • , Yi Zhang
    • , Matt Newgren
    •  & Jessica Finn

Supplement

United States & Canadian Academy of Pathology 109th Annual Meeting Abstracts: Eyes on You

“Security Retina Scanner on Blue Human Eye” Geebshot/iStock

United States & Canadian Academy of Pathology 109th Annual Meeting Abstracts: Eyes on You

The largest gathering of physician-pathologists in the world