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Association analysis of IL20RA and IL20RB genes in psoriasis

Genes & Immunity volume 9, pages 445–451 (2008)Cite this article

Abstract

The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. Its ligands are the three members of the IL19 subfamily of cytokines, IL-19, IL-20 and IL-24. These cytokines are important in the manifestation of psoriatic lesions and, recently, an association of polymorphisms of IL20 with psoriasis has been described. In the present study we tested the hypotheses that genetic variations of the IL-20-RI influence susceptibility to psoriasis and investigated single nucleotide polymorphisms (SNPs) in the IL20RA and IL20RB genes in psoriasis patients (n=254) and healthy controls (n=224). We found no association of any of the investigated SNPs with the disease. Analysis of pairwise linkage disequilibrium (LD) across studied markers revealed a strong level of LD between SNPs within the IL20RA gene and SNPs within the IL20RB gene, and, for both genes six common haplotypes were identified with an estimated frequency ⩾1%. Haplotype analyses suggested that the IL20RA haplotype CCG (rs1184860, rs1167846, rs1167849) is significantly associated with psoriasis (OR 3.14, 95% CI 1.61–6.14), whereas the TTG haplotype had a protective effect (OR 0.20, 95% CI 0.07–0.55). The risk haplotype defining SNPs 1167846 and 1184860 were found to modify paired box 5 and homeobox A9 sites, respectively, two transcription factors related to the differentiation of immune cells. Further studies are needed to confirm the genetic association and to investigate the functional relevance of IL20RA haplotypes in psoriasis.

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Acknowledgements

This study was financially supported by the target-based funding by the Estonian Ministry of Education grant no. SF0180043s07, University of Tartu research grant no. PARFS 05901, Estonian Science Foundation grant nos. 6576, 5712 and 7549 and by the Centre of Molecular and Clinical Medicine grant VARMC-TIPP.

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Authors and Affiliations

  1. Department of Dermatology and Venerology, University of Tartu, Tartu, Estonia

    K Kingo & H Silm

  2. Centre of Molecular and Clinical Medicine, University of Tartu, Tartu, Estonia

    K Kingo, R Rätsep, K Raud, E Vasar & S Kõks

  3. Department of Dermatology and Venerology, Georg-August-University Göttingen, Germany

    R Mössner & U Krüger

  4. Department of Physiology, University of Tartu, Tartu, Estonia

    R Rätsep, K Raud, E Vasar & S Kõks

  5. Dermatologikum, Hamburg, Germany

    K Reich

  6. Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia

    S Kõks

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  1. K Kingo
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  2. R Mössner
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  7. E Vasar
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  8. K Reich
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Correspondence to S Kõks.

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Kingo, K., Mössner, R., Rätsep, R. et al. Association analysis of IL20RA and IL20RB genes in psoriasis. Genes Immun 9, 445–451 (2008). https://doi.org/10.1038/gene.2008.36

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