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Notch-1 induction, a novel activity of SV40 required for growth of SV40-transformed human mesothelial cells

Oncogene volume 22, pages 81–89 (2003)Cite this article

Abstract

We show that SV40 infection of human mesothelial cells directly causes overexpression of Notch-1, a key cell regulatory gene. Notch-1 induction is achieved at the transcriptional level and requires both the SV40 large T-antigen and the small t-antigen. Notch-1 upregulation is maintained in SV40-transformed human mesothelial clones and in SV40-positive mesotheliomas and derived cell lines. Activation of Notch-1 promotes cell cycle progression and it is required for the growth of SV40-transformed mesothelial cells. Our finding is relevant to the process of SV40-mediated human cell transformation, an effect that cannot be accounted for solely by SV40-Tag inhibition of Rb and p53.

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Accession codes

Accessions

GenBank/EMBL/DDBJ

Abbreviations

SV40:

Simian virus 40

tag:

SV40 large tumor antigen

tag:

SV40 small tumor antigen

HM:

primary human mesothelial cells

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Acknowledgements

This work was supported by RO-1 CA92657, by the Charlotte Geyer Foundation, and by ACS RPG-99-238-01-CAN through a generous donation of Mr Dean Butckovitz, and by the Riviera Country Club, Orland Park, IL, to MC, and by RO-1 CA84065 to Lucio Miele. Lucio Miele provided expertise, reagents and advice for Notch-1 experiments. Harvey I Pass provided the mesothelioma biopsies.

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Authors and Affiliations

  1. Department of Pathology, Cancer Immunology Program, Cardinal Bernardin Cancer Center, Loyola University Chicago, Maywood, 60153, IL, USA

    Maurizio Bocchetta & Michele Carbone

  2. Department of Pharmacodynamics, and Pharmaceutics and Cancer Center, University of Illinois, Chicago, IL, USA

    Lucio Miele

  3. Aerodigestive Program, Karmanos Center Institute, Wayne State University, Detroit, 48201, MI, USA

    Harvey I Pass

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  1. Maurizio Bocchetta
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  2. Lucio Miele
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  4. Michele Carbone
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Correspondence to Michele Carbone.

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Bocchetta, M., Miele, L., Pass, H. et al. Notch-1 induction, a novel activity of SV40 required for growth of SV40-transformed human mesothelial cells. Oncogene 22, 81–89 (2003). https://doi.org/10.1038/sj.onc.1206097

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