Abstract
Genetic manipulation of insects and other arthropods may enable better control strategies to be developed against agricultural pests and disease vectors. Transposon-based transformation techniques have been implemented in Drosophila1 and other insects2 such as medflies3,4 and mosquitoes5,6. A major obstacle in the use of these transposons, however, has been the difficulty in obtaining marker genes that will allow easy and reliable identification of transgenic animals. Here we describe a marker system that is suitable for following gene transfer in most arthropods and in many other phyla.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Rubin, G. M. & Spradling, A. C. Science 218, 348–353 (1982).
O'Brochta, D. A. & Atkinson, P. W. Insect Biochem. Mol. Biol. 26, 739–753 (1996).
Loukeris, T. G. et al. Science 270, 2002–2005 (1995).
Handler, A. M., McCombs, S. D., Fraser, M. L. & Saul, S. H. Proc. Natl Acad. Sci. USA 95, 7520–7525 (1998).
Coates, C. J., Jasinskiene, N., Miyashiro, L. & James, A. A. Proc. Natl Acad. Sci. USA 95, 3748–3751 (1998).
Jasinskiene, N. et al. Proc. Natl Acad. Sci. USA 95, 3743–3747 (1998).
Tsien, R. Y. Annu. Rev. Biochem. 67, 509–544 (1998).
Sheng, G., Thouvenot, E., Schmucker, D., Wilson, D. S. & Desplan, C. Genes Dev. 11, 1122–1131 (1997).
Callaerts, P., Halder, G. & Gehring, W. J. Annu. Rev. Neurosci. 20, 483–532 (1997).
Warren, W. D., Atkinson, P. W. & O'Brochta, D. A. Genet. Res. 64, 87–97 (1994).
Cary, L. C. et al. Virology 172, 156–169 (1989).
Medhora, M. M., MacPeek, A. H. & Hartl, D. L. EMBO J. 7, 2185–2189 (1988).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Berghammer, A., Klingler, M. & A. Wimmer, E. A universal marker for transgenic insects. Nature 402, 370–371 (1999). https://doi.org/10.1038/46463
Issue Date:
DOI: https://doi.org/10.1038/46463
This article is cited by
-
Genome editing of the vermilion locus generates a visible eye color marker for Oncopeltus fasciatus
Scientific Reports (2023)
-
The red flour beetle T. castaneum: elaborate genetic toolkit and unbiased large scale RNAi screening to study insect biology and evolution
EvoDevo (2022)
-
Screens in fly and beetle reveal vastly divergent gene sets required for developmental processes
BMC Biology (2022)
-
A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
Scientific Reports (2021)
-
Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii
BMC Genetics (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.