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Genetic techniques

A universal marker for transgenic insects

Abstract

Genetic manipulation of insects and other arthropods may enable better control strategies to be developed against agricultural pests and disease vectors. Transposon-based transformation techniques have been implemented in Drosophila1 and other insects2 such as medflies3,4 and mosquitoes5,6. A major obstacle in the use of these transposons, however, has been the difficulty in obtaining marker genes that will allow easy and reliable identification of transgenic animals. Here we describe a marker system that is suitable for following gene transfer in most arthropods and in many other phyla.

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Figure 1: Transgenic fruitflies and flour beetles identified by the universal marker 3xP3-EGFP.

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Correspondence to Ernst A. Wimmer.

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Berghammer, A., Klingler, M. & A. Wimmer, E. A universal marker for transgenic insects. Nature 402, 370–371 (1999). https://doi.org/10.1038/46463

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