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Lipofection of early passages of cell cultures derived from murine adenocarcinomas: In vitro and ex vivo testing of the thymidine kinase/ganciclovir system

Cancer Gene Therapy volume 9, pages 96–99 (2002)Cite this article

Abstract

Early passages of cultured cells derived from four spontaneous Balb/c murine adenocarcinomas were used to explore the feasibility of a nonviral HSVtk-based suicide gene therapy system. After lipofection with pCMVtk, the transiently HSVtk expressing P07 (lung), M3, M05, and M38 (mammary gland) cells were, respectively, about 130-, 30-, 120-, and 170-fold more sensitive to ganciclovir (GCV) in vitro than their respective controls. Eighty percent of Balb/c mice subcutaneously inoculated with ex vivo pCMVtk-lipofected P07 cells, followed by intraperitoneal GCV injection for 7 days, displayed a complete inhibition of tumor growth for over 70 days. Control animals started to display tumors 13 days after inoculation. We present evidence showing that early passages of cultured tumor cells can efficiently express lipofected genes and that they are sensitive to the lipoplex-mediated HSVtk/GCV system.

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Acknowledgements

We thank G Sobrido for technical assistance, Dr. S Klein and Dr. M Diament for murine adenocarcinomas, BioSidus for the kind gift of DMRIE, and GCV. This work was supported by a grant from FONCYT: BID802/OC-AR-PICT 05-00037-00709 and CABBIO. ALK, GCG, and LMEF are members and VFB and CCC are fellows of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. GLF is a fellow of BioSidus S.A.

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  1. Armando L Karara and Viviana F Bumaschny: ALK and VFB equally contributed to this work.

  2. Gerardo C Glikin and Liliana ME Finocchiaro: On leave of absence from INGEBI-CONICET/FCEN-UBA.

Authors and Affiliations

  1. Unidad de Transferencia Genética, Instituto de Oncología “Ángel H. Roffo,” Universidad de Buenos Aires, Argentina

    Armando L Karara, Viviana F Bumaschny, Gabriel L Fiszman, Cecilia C Casais, Gerardo C Glikin & Liliana ME Finocchiaro

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  1. Armando L Karara
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  2. Viviana F Bumaschny
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  3. Gabriel L Fiszman
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  6. Liliana ME Finocchiaro
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Karara, A., Bumaschny, V., Fiszman, G. et al. Lipofection of early passages of cell cultures derived from murine adenocarcinomas: In vitro and ex vivo testing of the thymidine kinase/ganciclovir system. Cancer Gene Ther 9, 96–99 (2002). https://doi.org/10.1038/sj.cgt.7700417

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