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Engraftment

Similar myeloid recovery despite superior overall engraftment in NOD/SCID mice after transplantation of human CD34+ cells from umbilical cord blood as compared to adult sources

Bone Marrow Transplantation volume 28, pages 163–171 (2001)Cite this article

Abstract

Umbilical cord blood (UCB), bone marrow (BM) and mobilized peripheral blood (mPB) are used as sources of hematopoietic stem cells for transplantation. The NOD/SCID mouse model was used to compare the lineage-specific repopulating potential of CD34+ cells derived from these sources. Six to 8 weeks after transplantation, blood, BM, spleen, liver and thymus, were harvested, and analyzed by flow cytometry using CD34, CD45, myeloid, and lymphoid lineage-specific antibodies. Fifty percent engraftment of human cells in bone marrow of mice was estimated to be reached with 0.55 × 106 CD34+ UCB cells or with 7.9 × 106 CD34+ cells from adult sources, illustrating a 10-fold superiority of UCB CD34+ cells to engraft NOD/SCID mice. Lineage-specific characterization of engrafted human cells showed that the high engraftment potential of CD34+ cells from UCB was due to a preferential B cell development (2–81%). In contrast, comparable percentages of myeloid cells were found following transplantation of CD34+ cells from UCB, BM and mPB (1–38%), and occurred at significant levels only at relatively high doses. Since the CD34 content of UCB transplants is usually at least one log lower than of transplant from adult sources, these results correspond to the clinical findings with UCB transplantation showing a relatively high overall engraftment, but delayed myeloid recovery. Bone Marrow Transplantation (2001) 28, 163–171.

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Acknowledgements

We thank the midwives of the Department of Obstetrics and Gynaecology at the Bronovo Hospital, The Hague for the collection of cord blood samples. We are indebted to Helma Bauer for her support in collecting UCB samples, Dr Leo Ebeling for his support in collecting bone marrow samples and Janny van Nieuwkoop for her help to obtain peripheral mobilized blood samples. Hans Krohn, Jan Elk and Piet Bijl are acknowledged for taking care of the NOD/SCID mice. The work was supported by grant 9510–97 from the Dutch Cancer Society, The Netherlands.

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Authors and Affiliations

  1. Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands

    WA Noort, J Wilpshaar, CDP Hertogh, M Rad, EGA Lurvink, SAP van Luxemburg-Heijs, R Willemze & JHF Falkenburg

  2. Department of Obstetrics, Leiden University Medical Center, Leiden, The Netherlands

    J Wilpshaar

  3. Department of Medical Statistics, Leiden University Medical Center, Leiden, The Netherlands

    K Zwinderman

  4. Department of Obstetrics and Gynecology, Bronovo Hospital, The Hague, The Netherlands

    RA Verwey

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Noort, W., Wilpshaar, J., Hertogh, C. et al. Similar myeloid recovery despite superior overall engraftment in NOD/SCID mice after transplantation of human CD34+ cells from umbilical cord blood as compared to adult sources. Bone Marrow Transplant 28, 163–171 (2001). https://doi.org/10.1038/sj.bmt.1703120

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