Correction to: Scientific Reports https://doi.org/10.1038/s41598-018-19920-y, published online 29 January 2018
This Article contains an error in the order of the figure legends. The legends for Figures 4 and 5 were incorrectly given as those for 5 and 4 respectively.
The legend for Figure 4 should read:
“Growth curve of re-transformed auxotrophic E. coli strains in LB and M9 + glucose media. Each panel represents growth of cysteine-dependent E. coli auxotrophs rescued by wild type enzymes: CysE or CysM (blue), cysteine-free enzymes: CysE-C or CysM-C (orange), cysteine- and methionine-free enzymes: CysE-CM or CysM-CM (yellow), and LacZα protein expressed from original pUC19 plasmid (gray). Growth curve was monitored every 10 minutes at OD 600 nm using 96-well plate reader. Standard deviations of the growth curves are displayed as calculated from triplicates.”
The legend for Figure 5 should read:
“Synthetic cysE and cysM gene transformants display recovery of CysE function without cysteine and methionine and CysM function without cysteine. (A) E. coli ΔcysE competent cells were transformed with positive control cysE, two cysE variants cysE-C/cysE-CM cloned into the multiple cloning site of pUC19 plasmid, and original pUC19 encoding N-terminal fragment of lacZα as a negative control. (B) E. coli ΔcysMΔcysK competent cells transformed with positive control cysM, two cysM variants cysM-C/cysM-CM in pUC19 plasmid, and original pUC19 encoding N-terminal fragment of lacZα as a negative control. Cells were plated on M9 + glucose medium with 0.4 mM IPTG, 50 μg/ml kanamycin, and 100 μg/ml ampicillin and incubated at 30 °C for 72 h.”
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Fujishima, K., Wang, K.M., Palmer, J.A. et al. Author Correction: Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes. Sci Rep 9, 6242 (2019). https://doi.org/10.1038/s41598-019-42196-9
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DOI: https://doi.org/10.1038/s41598-019-42196-9
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