Addendum to: Nature Communications https://doi.org/10.1038/s41467-020-19689-7, published online 17 November 2020
In a recent paper1, we studied the allosteric mechanisms of a photoswitchable PDZ3 domain. The design of the system was motivated by previous works2, in which it has been shown that removing the terminal α3-helix of the PDZ3 domain, or disturbing its structure by phosphorylation, significantly affects the binding affinity of small peptides to the binding groove of the protein. We instead covalently linked a photo-isomerizable azobenzene moiety to the terminal α3-helix, designed in a way that the α-helical structure is stabilized in the cis-state of the photoswitch, and destabilized in the trans-state. This allowed us to reversibly switch between two states of the protein with the help of light. We showed by CD spectroscopy that the helical content of the protein indeed changed in an anticipated way (Fig. 2a in ref. 1), we measured the binding affinities of a small pentapeptide in the cis and the trans-state of the azobenzene moiety with the help of fluorescence quenching experiments (Fig. 2b in ref. 1), as well as the thermally driven cis-to-trans isomerization rates with and without a ligand bound to protein (Fig. 2c in ref. 1). From Van-t’Hoff and Arrhenius plots (Fig. 3 in ref. 1), the energetics of the allosteric cycle has been deduced and the ligand-induced force the protein exerts on the azobenzene moiety has been determined. The work contains two errors, one in the measurement of the binding affinities by fluorescence quenching, and a second conceptual one in determining the energetics. In this Addendum, we wish to address both errors.
Binding affinity measured by fluorescence quenching
In ref. 1, we measured the binding affinity by fluorescence quenching. The fluorescence originated from tryptophan in the pentapeptide ligand, whose yield increases when the peptide binds to the protein, presumably due to a more rigid structure. We kept the concentration of the peptide constant (15 μM), varied that of the protein between 0 and 50 μM, and fitted the resulting fluorescence signal to a two-state binding equilibrium. The excitation and detection wavelengths were set to 250 and 325 nm, respectively, both isosbestic points where the absorption of the azobenzene moiety is the same in the cis and the trans-states.
We, however, completely underestimated the effect of (re-)absorption. That is, as we increased the protein concentration in these experiments, its absorption due to the attached azobenzene moiety increased as well. This affects both the excitation of the tryptophan by absorbing excitation light at 250 nm, as well as the detection of its emission at 325 nm by reabsorption. The red data in Fig. 1 (which have been remeasured with different concentrations and different wavelengths as in ref. 1, see figure caption for details) illustrate the problem. In the cis-state, the fluorescence signal initially rises due to the reduced quenching upon binding, but then decreases again due to absorption effects. In the trans-state, the initial rise, which is smaller due to the smaller binding affinity, is overcompensated by the reabsorption effect already at low concentrations. In ref. 1, we stopped at a protein concentration of 50 μM, and thus wrongly fitted the maximum in the fluorescence signal at this point as the anticipated asymptotic value in a binding equilibrium. In that way, we significantly overestimated the binding affinities, as well as the differences between cis and trans-states. Furthermore, since all involved processes are temperature dependent in a competing manner, the resulting temperature trends were wrong.
We did not manage to reduce the sample thickness and/or sample concentration to the extent that the described absorption effects would be negligible; the amount of the resulting fluorescence light then was just too low to measure anything reasonable. Instead, we measured the fluorescence depolarization to determine the binding affinity3. The anisotropy is defined as (I∥ − I⊥)/(I∥ + 2I⊥)), where I∥ and I⊥ are the detected fluorescence signals for parallel and perpendicular polarization directions of excitation light vs emission detection, respectively (Fig. 1, red for parallel and blue for perpendicular polarization). The effects of absorption and reabsorption are canceled out in that way, since both happen in the bulk solution and thus do not have any polarization dependence. Since the unbound peptide is a relatively small molecule, it orientationally diffuses on the timescale of tryptophan fluorescence, and the detected anisotropy is smaller than the upper limit of 0.4. On the other hand, once it is bound to the protein, orientational diffusion does, in essence, no longer occur on this timescale due to the much larger size of the protein. Figure 2a shows the resulting anisotropies as a function of initial protein concentration and temperatures. A significant difference in binding between cis and trans-state can be observed, despite the fact that these data are more noisy than the raw fluorescence data of Fig. 1 (or those from Fig. 2b of ref. 1). They are more noisy since two data sets are put into relation with each other, and since the change in anisotropy between free and protein-bound peptide is very small. For the best possible comparability, trans and cis-states have therefore been measured directly after each other, for exactly the same sample and without touching anything except for switching on a 370 nm LED to promote the sample from the trans into its cis-state.
Fitting a two-state binding equilibrium to the data, the binding affinities can be extracted, which are listed in the upper half of Table 1. In optimizing the measurement parameters, one critical issue has been to be able to measure at a protein concentration high enough, despite reabsorption, so that the plateau of full binding is reached; otherwise, the fit would not have been stable. The concentrations that were needed to obtain a sufficient amount of fluorescence are significantly larger than the dissociation constant for the cis-state, hence its error is relatively large. Nonetheless, it is clear that the binding affinity of the cis-state is significantly larger at all temperatures by about a factor of 8 ± 2, which amounts to a binding free energy that is larger by \(\Delta \Delta G=RT\ln {K}_{d,cis}/{K}_{d,trans} \, \approx \, 5.2\pm 0.5\) kJ/mol.
Determination of the energetics
Besides the error in the data accumulation described above, there has also been a conceptual mistake in the data analysis. That is, we used the temperature-dependent binding affinities to disentangle the binding free energy, ΔG, into its enthalpic and entropic contributions via ΔG = ΔH − TΔS. To that end, we implicitly assumed that ΔH is temperature independent and that the temperature dependence of ΔG is dominated by the explicit T-factor in the equation above. That assumption is inherently wrong for a protein-ligand association, as discussed in a number of publications.4,5,6
In fact, in order to verify the new binding affinities measured by fluorescence depolarization (Fig. 2a), we also used ITC as an alternative method, see Fig. 2b. In contrast to fluorescence depolarization, which reveals only the binding free energy, ITC measures ΔH (i.e., the explicit measurand of ITC) and ΔG (via the slope of the data in Fig. 2b) separately for each individual temperature, see Table 1, lower half. ITC is the only method that can measure ΔH of binding directly and independently from ΔG, and hence does not have to rest on any assumption. We find that the binding enthalpy, ΔH, and the entropic contribution, − TΔS, are strongly temperature dependent, albeit in a way that the temperature dependence of the binding free energy, ΔG, largely cancels out in the considered temperature range (Fig. 3). This effect is known as “entropy-enthalpy compensation”.4,5,6 To a certain extent accidentally, the linear term of both contributions cancel out, but higher order terms remain, hence the resulting free energy ΔG is curved in the considered temperature range (see e.g., Fig. 3 in ref. 4).
Figure 3 also compares the binding free energy determined from ITC (in blue) with that determined with the help of fluorescence depolarization (in green), revealing a good agreement. Unfortunately, we cannot investigate the cis-state with ITC, as that would require having the photoswitchable protein as the titrant in the syringe at mM concentrations, constantly illuminated during the experiment. Nevertheless, the good agreement of the trans-data gives us confidence that the cis-data of Fig. 2a and Table 1 are qualitatively correct as well.
Consequences for the conclusions
The CD data in Fig. 2a of ref. 1, as well as the kinetic data in Figs. 2c, 3b of ref. 1 are not affected by the measurement error, since the protein and peptide concentrations, and hence the effect of reabsorption, are kept constant in either case. On the other hand, Fig. 2b of ref. 1 is wrong and has to be replaced by the new Fig. 2a presented here. There is no swap of binding affinity between cis and trans-state as a function of temperature, rather, the temperature dependence is weak (Fig. 3, blue and green). The cis-state has an about 8 ± 2 times larger binding affinity at all temperatures, which is still a sizeable effect, e.g., is an effect larger than that upon phosphorylation of the α3-helix.2 The Van-t’Hoff plot in Fig. 3a of ref. 1 is wrong and has to be replaced by Fig. 3, presented here.
Figure 4 replaces Fig. 4c of ref. 1. However, since we cannot disentangle enthalpic vs entropic contributions of the cis-state, Fig. 4 plots free energy profiles for the thermal cis-to-trans isomerization, rather than the enthalpy profiles in ref. 1. Since the binding energy is larger in the cis-state, the free energy driving force for isomerization is smaller in the ligand-bound state PL, yet the kinetics in this state is faster (see Figs. 2c, 3b of ref. 1). In the language of a Φ-value analysis, which we used in ref. 1, that situation results in a negative Φ-value. The other prominent example in this regard is the “inverted regime” of electron transfer. Both situations are relatively rare, but not unheard of7,8,9. It typically implies that the reaction does not proceed in a straightforward manner along a particular reaction coordinate. For example, in the inverted regime of electron transfer, the reaction first moves “backward” along a collective solvation coordinate until it reaches the transition state, from where it then proceeds forward toward the product state. In the concrete case here, it implies that the isomerization coordinate of the azobenzene moiety is orthogonal to that of ligand binding. Nonetheless, the Arrhenius plot in Fig. 3b of ref. 1 reveals that for temperatures <40 °C, the thermally driven cis−trans isomerization is faster with the ligand bound, hence, the reaction barrier against isomerization is lower in terms of free energy, according to an Eyring equation, \(k={k}_{B}T/h\exp (-\Delta {G}^{\#}/{k}_{B}T)\). While we no longer attempt to quantify the size of the force that gives rise to this effect, the results show that such an allosteric force exists.
Most importantly, the conclusions on bi-directionality of allosteric control remains, i.e., ligand binding affects the “reactivity” of the azobenzene moiety, and vice versa, the configuration of the azobenzene moiety significantly affects the binding affinity of the ligand. It is thus the smallest truly allosteric protein system, that, for example, is accessible to full-atom molecular dynamics simulations due to its small size and thus promises new insights into the microscopic understanding of allosteric communication. In summary, while many of the numbers in ref. 1 are wrong, the essential conclusions of the paper, i.e., bi-directional allosteric control, including the ability to sense the allosteric force, remain correct.
References
Bozovic, O., Jankovic, B. & Hamm, P. Sensing the allosteric force. Nat. Commun. 11, 5841 (2020).
Petit, C. M., Zhang, J., Sapienza, P. J., Fuentes, E. J. & Lee, A. L. Hidden dynamic allostery in a PDZ domain. Proc. Natl. Acad. Sci. USA 106, 18249–18254 (2009).
Lakowicz, J. R. Principles of Fluorescence Spectroscopy 3rd edn (Springer, 2006).
Cooper, A., Johnson, C. M., Lakey, J. H. & Nöllmann, M. Heat does not come in different colours: entropy-enthalpy compensation, free energy windows, quantum confinement, pressure perturbation calorimetry, solvation and the multiple causes of heat capacity effects in biomolecular interactions. Biophys. Chem. 93, 215–230 (2001).
Reynolds, C. H. & Holloway, M. K. Thermodynamics of ligand binding and efficiency. ACS Med. Chem. Lett. 2, 433–437 (2011).
Fox, J. M., Zhao, M., Fink, M. J., Kang, K. & Whitesides, G. M. The molecular origin of enthalpy/entropy compensation in biomolecular recognition. Annu. Rev. Biophys. 47, 223–250 (2018).
De Los Rios, M. A., Daneshi, M. & Plaxco, K. W. Experimental investigation of the frequency and substitution dependence of negative ϕ-values in two-state proteins. Biochemistry 44, 12160–12167 (2005).
Ozkan, S. B., Bahar, I. & Dill, K. A. Transition states and the meaning of Φ-values in protein folding kinetics. Nat. Struct. Biol. 8, 765–769 (2001).
Marcus, R. A. Electron transfer reactions in chemistry. Theory and experiment. Rev. Mod. Phys. 65, 599–610 (1993).
Acknowledgements
The work has been supported by the Swiss National Science Foundation (SNSF) through Grant 200020B 188694/1.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Jankovic, B., Bozovic, O. & Hamm, P. Addendum: Sensing the allosteric force. Nat Commun 14, 2735 (2023). https://doi.org/10.1038/s41467-023-37893-z
Published:
DOI: https://doi.org/10.1038/s41467-023-37893-z
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.