Abstract
Purpose
Panel testing has led to the identification of TP53 pathogenic/likely pathogenic (P/LP) variant carriers (TP53+) who exhibit a broad range of phenotypes. We sought to evaluate and compare genotype–phenotype associations among TP53+ panel-ascertained subjects.
Methods
Between 2012 and 2017, 317 TP53+ subjects (279 females and 38 males) identified through panel testing at one testing laboratory were found to have evaluable clinical histories and molecular results. Subject cancer histories were obtained from test requisition forms. P/LP variants were categorized by type and were examined in relation to phenotype.
Results
Loss-of-function (LOF) variants were associated with the earliest age at first cancer, with a median age of 30.5 years (P = 0.014); increased frequency of a sarcoma diagnosis (P = 0.016); and more often meeting classic LFS testing and Chompret 2015 criteria (P = 0.004 and 0.002 respectively), as compared with dominant-negative missense, other missense, or miscellaneous (splice or in-frame deletion) P/LP variant categories.
Conclusion
Loss-of-function variants were more often associated with characteristic LFS cancer histories than other variant categories in TP53+ carriers ascertained through multigene panel testing. These findings require validation in other TP53+ cohorts. Genetic counseling for panel-ascertainedTP53+ individuals should reflect the dynamic expansion of the Li–Fraumeni syndrome phenotype.
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INTRODUCTION
Despite its original description by Drs. Li and Fraumeni nearly 50 years ago, many questions about the phenotype of Li–Fraumeni syndrome (LFS) remain.1 LFS is a pleiotropic hereditary cancer predisposition syndrome associated with germline pathogenic/likely pathogenic (P/LP) variants in the TP53 gene.2 Classically it has been associated with rare malignancies, aggressive cancers in children and adults at early ages, and multiple primary tumors, with a particular propensity for sarcoma, brain/central nervous system (CNS) tumor, breast cancer, and adrenocortical carcinoma.1,3,4
As multigene panel testing for hereditary cancer has gained traction, an increasing number of germline TP53 P/LP variants have been identified in individuals who do not meet established criteria for LFS. We previously described differences between multigene panel–ascertained TP53 P/LP variant (TP53+) carriers with single-gene tested TP53+ subjects. Panel-based TP53+ subjects were diagnosed with their first cancer at later ages than single-geneTP53+ individuals and were significantly less likely to meet Chompret or National Comprehensive Cancer Network (NCCN) criteria forTP53 testing.5 These findings suggested more phenotypic variability in germline TP53+ subjects than previously appreciated.
In parallel, questions have arisen about the higher than expected frequency of germline TP53 P/LP variants in cohorts of patients with breast, colorectal, and other cancers.6,7,8,9,10 Efforts to reevaluate the epidemiology of LFS were recently undertaken through analysis of tumor types by phase of life (childhood, early adulthood, and later adulthood) by retrospective review of the International Agency for Research on Cancer (IARC) TP53 database.11 Likewise, colleagues have recognized that stringent TP53 testing guidelines miss individuals with germline TP53+ results and that the understanding of germline TP53 P/LP variants would be aided by less discriminant TP53 testing.12 Complicating matters, however, is the identification of TP53 P/LP variants due to clonal hematopoiesis of indeterminate potential (CHIP), which can lead to misclassification and misdiagnosis.13 Missense variation in the TP53 gene poses another longstanding challenge to diagnosing LFS, which has resulted in renewed efforts to improve in silico prediction tools for interpretation of these variants.14
An unanticipated TP53+ result on panel testing can be alarming for patients and clinicians alike. There is sustained interest in genotype–phenotype associations in TP53+ carriers including effects of loss-of-function or null, hotspot, founder, missense, and oligomerization domain (residues 325–355) P/LP variants.4,15,16,17,18 To aid in risk assessment and genetic counseling, we examined the clinical characteristics and investigated genotype–phenotype associations among TP53+ subjects ascertained through multigene panel testing (MGPT).
MATERIALS AND METHODS
Subject and phenotype analysis
The study cohort was limited to subjects with a TP53+ result, defined as a P/LP variant, who also had evaluable clinical and molecular information. Subject specimens were submitted to a single laboratory (Ambry Genetics, Inc.) between March 2012 and June 2017 for panel testing including TP53. Twelve different panels yielded at least one positive result (Supplemental Table 1). This cohort is an expansion of a previously reported cohort in which the distribution of TP53+, variant of uncertain significance (VUS), and negative results by clinical phenotype and test type was described.5
Subjects’ cancer histories were obtained from clinician-completed test requisition forms (TRFs), which accompany all specimens, along with additional clinical documents such as pedigrees and/or clinic notes when available. Each TP53+ subject’s clinical history was also reviewed to determine if Chompret criteria (2.2017) or classic LFS criteria were fulfilled.3,4,19,20 LFS core cancers in this study were defined as sarcoma, CNS tumor, and adrenocortical carcinoma. Breast cancer, while a classic LFS component tumor, was examined separately because of its high frequency in the cohort.
Dana-Farber/Harvard Cancer Center’s (DF/HCC) Institutional Review Board (IRB) and Western IRB provided oversight in accordance with regulations. The sharing of de-identified data was determined to be exempt from the Office of Human Research Protection’s Regulations for the Protection of Human Subjects.
Molecular analysis
Sequence enrichment, next-generation sequencing (NGS), targeted chromosomal microarray, and bioinformatics were performed, as previously described, of all coding exons and 5′ base pairs into introns.21,22 Variant classification has been previously described and was based on the variant interpretation as of April 2018.23,24TP53+ results were categorized as follows: loss-of-function including genomic rearrangement (LOF), missense as either dominant-negative (DN) missense effects per the IARC TP53 Database (http://p53.iarc.fr) or other missense (OM),25,26,27 and miscellaneous (MISC) including splice variants and in-frame deletions.
Statistical analysis
Descriptive statistics for subjects stratified by TP53+ category (LOF, DN, OM, and MISC) are summarized as median (interquartile range [IQR]) for continuous and proportions for categorical characteristics. Differences in medians and proportions across variant category were assessed with Kruskal–Wallis tests and Fisher’s exact tests, respectively. Frequencies of specific P/LP variants were summarized by phenotype group: subjects with a personal history of only breast cancer, subjects with a history of sarcoma alone or in combination with any other cancer, and all other subjects. Fisher’s exact test was used to determine whether the proportion of subjects in each phenotype group differed by P/LP variant type (LOF, DN, OM, and MISC). All analyses were conducted with R v.3.3.3.
RESULTS
Cohort definition
Of the 468 subjects with heterozygous TP53+ results from panel testing, 141 subjects (30%) were excluded from genotype–phenotype analysis based on a TP53 mosaic result defined as either allelic frequency <25% on NGS and/or clinical history or molecular results suspicious for somatic interference. Additional exclusions were as follows: 5 (1.07%) had a knownTP53+ result prior to MGPT, 4 (0.85%) had an unknown personal cancer history, 1 (0.21%) had two TP53 P/LP variants and whether they were in cis or trans was unknown. Thus, there were 317 subjects with TP53+ results with evaluable clinical and genetic information who were ascertained from panel testing.
TP53+ subjects with a second positive result (P/LP variant) in another cancer predisposition gene were included in the analysis (n = 21, 6.6%):ATM (4.8%), BRCA1 (14.3%), BRCA2 (4.8%),BRIP1 (4.8%), CHEK2 (9.5%), MRE11A (4.8%),MSH2 (4.8%), MSH6 (4.8%), PALB2 (4.8%),PMS2 (9.5%). Known low penetrance risk alleles were present in seven subjects (33.3%): four MUTYH heterozygotes and three APC, p.I1307K carriers (Supplemental Table 2). Sensitivity analysis was performed on all significant outcomes including and excluding these subjects and the results were similar (data not shown).
Demographics
Of 317 phenotype-evaluable TP53+ subjects, 279 (88%) were female and 38 (12%) were male (Table 1). The TP53+ subjects were of the following ancestries: Caucasian (64.4%), Hispanic (8.5%), African American/Black (7.6%), Asian (7.3%), and Other (12.3%). Most subjects (90.9%) had a personal history of cancer. Multiple primary tumors were noted in 89 subjects (28.1%). The median ages (IQR, range) at first cancer diagnosis and genetic testing were 36 (19, 1–77) years and 45 (22, 5–90) years respectively. Only five of the TP53+ subjects (1.5%) were under age 18 at the time of testing. While 214 female subjects (67.5%) had breast cancer, it was the first malignancy in 181 female subjects (57.1%). None of the 38 males were known to have breast cancer.
P/LP variant spectrum
Among the 317 subjects, there were 133 distinct TP53 P/LP variants identified, of which 95 (71.4%) appeared in this cohort only once. All P/LP variants (Supplemental Table 3) were uploaded to ClinVar (https://www.ncbi.nlm.nih.gov/clinvar/). The frequencies of each P/LP variant in this cohort, in the IARC TP53 database, and in gnomAD are provided, but due to small numbers and differences in ascertainment, no direct comparisons were made. The distribution of variant types was as follows in decreasing frequency: 165 dominant-negative missense, 75 other missense, 57 LOF (14 genomic rearrangements, 22 nonsense, 21 frameshifts), and 20 miscellaneous variants (17 splice, 1 indel, and 2 in-frame deletions) (Table 1).
Cancer phenotype by variant category
The median age at first cancer diagnosis and age at testing significantly differed by P/LP variant type, with LOF carriers having the earliest age at first cancer diagnosis, median 30.5 (16.25) years; P = 0.014, and in turn the earliest age at testing, median 36 (24) years; P = 0.008. Median age at first cancer diagnosis of LOF carriers was 6–8 years earlier, and median age at testing was 10 years earlier than respective median ages for carriers of non-LOF variant types. LOF carriers were also significantly more likely than non-LOF carriers to meet classic LFS (8.8%; P = 0.004) or Chompret 2015 (56.1%; P = 0.002) testing criteria and to have an LFS core cancer without breast cancer (29.8%; P = 0.043). P/LP variant category was not associated with the development of multiple primaries.
Sarcoma was significantly more common in subjects with LOF variants (28.1%, P = 0.016) than in subjects with other variant types (Table 2, Fig. 1). There were no significant differences in the prevalence of many cancers by P/LP variant category including other classically associated LFS cancers (adrenal, brain, breast, leukemia), melanoma, and cancers of the ovary, pancreas, prostate, thyroid, and uterus. The prevalence of cancers of the biliary tract and colorectum also did not differ by variant category, but gastric cancer was associated with LOF P/LP variants (P = 0.01). Four of five subjects with gastric cancer had additional malignancies.
Within specific cancers, variant category was generally not associated with median age at diagnosis, although the variant-specific sample size was too small to assess significance for most cancer types. The median (IQR) age of breast cancer diagnosis overall was 38 (17) years. While breast cancer median (IQR) age was earliest in LOF subjects (33 [15] years), this was only marginally lower than the median age at breast cancer diagnosis in other P/LP variant types (p = 0.07). When limiting analysis to breast cancer as the first cancer diagnosis and stratifying by age at diagnosis at 35 years, LOF variant carriers were more often diagnosed at ≤35 years compared with non-LOF carriers (p = 0.042; Table 2).
Cancer phenotypes by P/LP variant category were evaluated in aggregate, mutually exclusive groupings (Table 3): breast cancer only, sarcoma regardless of another cancer, and not otherwise specified (NOS). The NOS category contained 29 (23%) subjects with no personal history of cancer, 41 (32.5%) with breast and an additional cancer exclusive of sarcoma, 9 (7.1%) with ovarian cancer alone, 9 (7.1%) with colorectal cancer alone, and 38 (30.2%) with unique combinations of one or more other cancer phenotypes. Of 146 subjects with a breast cancer only, 76 (52.1%) had dominant-negative missense P/LP variants, 38 (26%) had other missense P/LP variants, 25 (17.1%) had LOF P/LP variants, and 7 (4.8%) had miscellaneous P/LP variants (Table 3). P/LP variant category was not significantly associated with the aggregate cancer phenotype (p = 0.058). However, dominant-negative missense P/LP variants accounted for 52.1% of the cases with breast cancer only and were followed distantly by the other P/LP variant categories: other missense variants (26%), LOF (17.1%), and miscellaneous (4.8%).
Recurrent P/LP variants
There were 24 P/LP variants identified four or more times in this cohort, accounting for 191 subjects. We also report the above aggregate, mutually exclusive cancer phenotype for these specific P/LP variants (Supplemental Table 4). The two most common recurrent P/LP variants, c.467G>A (p.R156H) and c.743G>A (p.R248Q), were each identified 17 times and there were not specific patterns of cancers associated with them. The c.473G>A (p.R158H) variant was identified 6 times, all of which were in breast cancer only cases. Except for c.1010G>A (p.R337H), none of the recurrent P/LP variants were in the TP53 oligomerization domain. The whole-gene deletion, 5′UTR_3′UTRdel, was not identified among subjects with a personal history of breast cancer only, but instead was present in subjects with sarcoma (with or without other cancers).
DISCUSSION
Our data demonstrate considerable phenotypic heterogeneity amongTP53+ subjects ascertained through panel testing. Compared with other P/LP variant categories, LOF variants were associated with the earliest ages of cancer diagnosis overall, and with sarcoma. Despite this, the P/LP variant category was not associated with age of onset within each cancer type. Thus, the P/LP variant category is not yet a consistent means to distinguishTP53+ carriers with more classic, high penetrance LFS from the less striking phenotype, which is critical for genetic counseling purposes.
The association of LOF variants with the earliest-onset cancers is consistent with findings from a previous US-based cohort ascertained through childhood cancers.18 An IARC-based analysis also found that missenseTP53 P/LP variants representing partial deficiency transactivation alleles were associated with a milder phenotype of LFS compared with loss-of-function variants, which ostensibly represent severe deficiency due to protein truncation.16,28 These results differ from findings among the French LFS cohort, in which DN missense variants in which the mutant transcript interferes with the transcriptional activity of the wild-type p53 were associated with earlier onset of malignancies.4,29
We note the age of onset of breast cancer (38 years) in this cohort is older than typically associated with Li–Fraumeni syndrome. While two-thirds of LOF variant carriers diagnosed with breast cancer as their first cancer were diagnosed under age 35, non-LOF P/LP variants were less frequently associated with a breast cancer diagnosis below age 35 years. Our recent publication found that the median age of breast cancer in TP53+ carriers from panel testing was 40 years and 22% of TP53-associated breast cancers were diagnosed after age 45 years.5 These findings are remarkably consistent with findings from two adult cancer genetics services in Ireland, in which 18% of the breast cancers among TP53+ women were diagnosed after 49 years.12 Together these data suggest that re-evaluation of age-specific penetrance of breast cancer in TP53+ carriers is required as is evaluation of the role of family history, hormones, and polygenic risk factors.
The incidence of ovarian cancer among TP53+ subjects has been poorly quantified due to the rarity of this syndrome and likely also due to competing comorbidities. While the frequency of ovarian cancer among this cohort (8.6%) is appreciably higher than in another reported TP53+ cohort (1%), this likely reflects the differences in ascertainment of each cohort.4 Nonetheless, this bears investigation in other TP53+ cohorts.
Our findings must be interpreted in the context of the study constraints. Phenotype analysis was limited to the subject’s cancer phenotype. Thus genotype–phenotype correlations did not account for family cancer history. It was prudent to limit our analysis to the subject’s phenotype because this is where the most detailed, high-quality phenotypic information is available.30 We have previously demonstrated that among LFS families, cancers are underreported and misreported owing to the rare cancer types in the syndrome.31 Our data are from mostly an adult, cancer-affected population, half of whom were women diagnosed with breast cancer as their first malignancy, which is reflective of the majority of probands referred for cancer genetic testing. Until recently, children suspected to have LFS were not tested with multigene panels, but rather through TP53 single-gene testing;32,33,34 thus it is possible that severe genotypes are underrepresented or not present in this cohort due to testing practices as well as survivor effects. The disproportionate cancer genetic testing of adult women with breast and/or ovarian cancer, while guideline-based, limits the generalizability of our findings.19 While exclusions were made to account for cases with somatic interference or clonal hematopoiesis of indeterminate potential, we could not systematically exclude it through tissue analysis in all cases. Finally, we note that the variant categories (LOF, DN missense, other missense, and miscellaneous) provide a framework for analyzing the detected variation in TP53. However, these categories do not represent absolutes as some LOF variants may retain expression of forms of p53 and some missense variants maintain partially functional transactivation.
There were too few P/LP variants identified in the oligomerization domain (OD), which affect the tetramerization of p53, to perform genotype assessment by oligomeric status of the resultant p53 proteins. Interestingly, colleagues recently demonstrated that carriers with mutant multimeric p53 had significantly favorable survival compared with carriers of OD variant resulting in monomeric p53.15 Monomeric p53 has been associated with the greatest reduction in p53 transcriptional activity.15 This study highlights the importance of residue- and allele-specific functional analysis to inform the effects of genotype on phenotype.
One recent study of “hotspot” TP53 somatic variants suggests that cancer cell lines with these variants did not demonstrate TP53 dependency and may occur for other reasons including spontaneous deamination of the cytosine residue.35 Likewise, a new American College of Medical Genetics and Genomics (ACMG)–approved in silico prediction tool for analysis ofTP53 missense variants may help to reconcile the challenges associated with TP53 variants of uncertain significance.14
The extent to which TP53+ individuals identified through panel testing or their families face classic LFS cancer risks remains unknown. The challenge of counseling and managing cancer risk in nonclassic, forme fruste families found withTP53+ genetic test results is likely to increase. Careful characterization and prospective follow-up of panel-ascertainedTP53+ carriers and their families are required to answer the most important questions. In the interim, there are measures that may help in evaluating the full spectrum of a molecular TP53 diagnosis. Ideally, screening studies would have sufficient power to account for family history of LFS (classic vs. nonclassic) in assessing outcomes of surveillance protocols until a more personalized approach becomes available, taking into account modifier genes and other molecular markers of LFS severity.36 Case-level de-identified data sharing ofTP53+ subjects with long-term follow-up to measure outcomes, and incidence of interference from mosaicism or CHIP, will be necessary as germline TP53+ results continue to be a rare finding, although with urgent clinical implications.
It seems prudent that counseling of TP53+ subjects reflect the breadth of observed phenotypes and published risk estimates be contextualized with the bias of years of limiting testing to classic and other highly suggestive LFS families. Updated accurate cancer risk estimates will require long-term, multigenerational, prospective data collection on kindreds ascertained through panel testing. In the absence of validated individualized cancer risk data, clinical management of all germlineTP53+ subjects should utilize currently recommended multifaceted protocols such as those published by NCCN or the American Association for Cancer Research (AACR).19,37,38
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J.C., L.H., H.L., M.H.B., S.L., K.M., V.S., J.S.D., and C.-L.G. are employees of Ambry Genetics, Inc. J.E.G. has received research support from Myriad Genetics and consulting fees from Helix; her spouse has received research support and consulting fees from Novartis Oncology, and consulting fees from GTx. H.Q.R. declares no conflicts of interest.
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Rana, H.Q., Clifford, J., Hoang, L. et al. Genotype–phenotype associations among panel-based TP53+ subjects. Genet Med 21, 2478–2484 (2019). https://doi.org/10.1038/s41436-019-0541-y
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DOI: https://doi.org/10.1038/s41436-019-0541-y
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