Abstract 12
Background: Free oxygen radicals may have a role in hypoxic cell death and have been studied in various models. However, there may be species differences which make a study in human neurons interesting.
Materials & Methods: Human NT2 teratocarcinoma cells were differentiated to NT2-N neurons and exposed to hypoxia for 6 hours. Drugs were added immediately before hypoxia. Cell death was evaluated after 24 hours by LDH release. LDH release was normalized to untreated hypoxic wells in each experiment and presented as mean and standard deviation (ANOVA with Dunnetts post-hoc test).
Results: Both the water-soluble vitamin E-analogue trolox(p<0.05) and the flavonoid rutin (p<0.01) reduced LDH release at 24 hours significantly compared to untreated wells. These effects were however markedly less pronounced than the effect of the NMDA receptor blocker MK-801.Table
Conclusion: A limited but significant effect of antioxidants on LDH release after hypoxia may indicate a role for free oxygen radicals in hypoxic cell death in human NT2-N neurons.
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Almaas, R., Saugstad, O., Pleasure, D. et al. The antioxidants trolox and rutin protect human NT2-N neurons during hypoxia. Pediatr Res 44, 421 (1998). https://doi.org/10.1203/00006450-199809000-00045
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DOI: https://doi.org/10.1203/00006450-199809000-00045
