The prediction trials for IDDM requires the evaluation of the predictive value of autommune markers in newly onset diabetic patients. The aim of this study was to determine the prevalence, sensibility and specificity of the antibodies ICA A-GADIAA and PAA in patients with recent onset diabetes evaluated from june 1994 to july 1995.

We studied 32 patients with IDDM,17 males and 15 females, and 32 controls matched by sex and age, without personal or familial history of autoimmune disease. The mean age of the patients was 11.03 DS +4.3 yr vs 11.00 DS+4.1 yr in control children.

The determinations wer e done in fasting serum samples obtained from every child within 72 hours after initiation of unsulin therapy IAA and PAA were detected by radiobanding assay (RBA)A-GAD by immunoprecipitation with human recombinant35 S metionyl GAD expressed in reticulocytes and ICA by indirect immunotluroesence using a single human group O pancreas.

The results are shown in the table:

Table 1
Full size table

We also detected the following antibodies in this study: IAA in 34.4% and PAA in 13.3% of patients.

In the group of ICA +diabetic children.37.5% had between 10 and 40 JDF U.21% between 40 and 80 JDF U and 41.6% more than 80 JDF U. The prevalence of ICA+ and A-GAD + was 50%, ICA + and IAA + 28% and ICA+ and A-GAD + and IAA+ was 18.7%. At least one marker was found in 93.7% of the study population.

There was no correlation between clinical patherns of onset, age time of the year and specific status antibody Ours results are consistent with the reported results.

We suggest that strategues combining multiple immunological markers woul improve the prediction values in tirs degree relatives, final goal of our study.