Dietary FO rich in w-3 polyunsaturated fatty acids (PUFA) has been proposed to slow down the porgression of renal disease. Since PUFA are highly susceptible to lipid peroxidation, FO may trigger oxidative tissue damage. To evaluate this hypothesis, we compared the effects of standard chow diet (CH) with a diet enriched with 20% menhadeniol (FO) in 40 male Sprague-Dawley rays weighing 250-300 gms. Half of the rats were made nephrotic afte IP injection of puromycin aminonucleoiside (PAN) 15 mg/100 g B.W., and the other half received IP normal saline (SAL). While on the same diet, injections were repeated at day 30 and were sacrificed at day 90. Serum creatinine, albumin, total cholesterol and urinary protein were measured at 0,45, and 90 days. Renal histopathology was analysed at the end of the study. Lipid peroxidation was measured by conjugated dienes formation using spectroscopic analysis of plasma and kidney lipid extracts. Results are expressed as mean +/- SD.Table Nephrotic rats on FO had significant improvement in proteinuria, hypercholesterolemia and hypoalbuminemia, although serum creatinine and histopathological findings were not different between both nephrotic groups. Conjugated dienes were not detected either in plasma or kidney tissue. We conclude that FO diet has a beneficial effect on nephrotic rats without evidence of abnormal lipid peroxidation.

Table 1
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