Abstract
ELISA microtiter plates ( Greiner ) were coated with highly purified bovine α-caseins and incubated with diluted sera. Specific IgE was detected by successive incubation with three antibodies, the last one conjugated with horseradish peroxidase. Twelve patients ( age 1/2 - 3 years ) with positive provocation and skin prick test to bovine caseins were compared with 12 children ( age 1/2 -12 years ) with negative tests. The results were compared to CAP-RAST ( Code f78, Pharmacia ) data. Additionally IgE-immunoblots were made as a qualitative criterion and to distinguish between immunreactions to the different caseins and the other major cow milk proteins.
67% of the cow milk allergic patients had positive RAST ( class > 1 ) and 92% were positive ( OD > 0.1 ) in the ELISA. In the immunoblot 83% showed distinct reactions to α-caseins. None of the control group gave positive reactions. Only one child's serum showed a positive reaction to α-caseins in the immunoblot.
In conclusion, compared with the clinical allergy of the children studied our ELISA proved to have a higher sensitivity than the RAST; specitivity of both tests equalled 100%.
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Spürgin, P., Walter, M., Müller, H. et al. 217 EVALUATION OF AN IGE-ELISA SPECIFIC FOR BOVINE α-CASEINS. Pediatr Res 36, 39 (1994). https://doi.org/10.1203/00006450-199407000-00217
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DOI: https://doi.org/10.1203/00006450-199407000-00217