Abstract
ABSTRACT: We compared oxygen-related prostaglandin synthesis in fetal lamb ductus arteriosus (DA) pulmonary artery (PA) and aorta endothelial and smooth muscle cells. We measured basal synthesis of 6-keto-PGF1α and PGE2, the response to calcium ionophore (A23187), a nonspecific stimulus of prostaglandin production, as well as the response to oxygen, a perinatal stimulus, monitoring both the effects of hyperoxia (95% O2) and hypoxia (2% O2). In addition, we established whether differences observed in fetal lamb PA cells related to oxygen tension were also observed in newborn central and microvessel PA cells. Our results indicate that DA endothelial cells increase 6-keto- PGF1α in response to ionophore (p<0.05). With hyperoxia, DA endothelial cells increase PGE2 synthesis and DA smooth muscle cells increase 6-keto-PGFla (p<0.05 and 0.02, respectively). Aorta smooth muscle cells increase 6-keto-PGFia in response to ionophore and hyperoxia (p < 0.003 and 0.05, respectively). PA endothelial and smooth muscle cells have higher levels of basal prostaglandin synthesis when compared with DA and aorta. In response to ionophore, increased 6-keto-PGF]a is observed in both PA endothelial and smooth muscle cells (p<0.02 and 0.0004, respectively), and PGE2 is increased in PA smooth muscle cells (p<0.003). Hypoxia, however, decreases PA smooth muscle production of both 6-keto-PGFia and PGE2 (p<0.02 and 0.01, respectively). Similar observations were made in newborn lamb central and microvessel PA cells. Thus, the hyperoxia-induced increase in prostaglandin synthesis in DA (endothelium and smooth muscle) and aorta (smooth muscle) and the hypoxia-induced decrease in prostaglandin synthesis in PA (smooth muscle) seem to be phenotypic properties of the cells in culture.
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Rabinovitch, M., Boudreau, N., Vella, G. et al. Oxygen-Related Prostaglandin Synthesis in Ductus Arteriosus and Other Vascular Cells. Pediatr Res 26, 330–335 (1989). https://doi.org/10.1203/00006450-198910000-00009
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DOI: https://doi.org/10.1203/00006450-198910000-00009